Recent References on Cell Proliferation Studies Using
ALZET^® Osmotic Pumps

1. BrdU (2000-2007)

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2. Thymidine (Studies From 1996-2007)

P7532 Helander,H.F., Li,H. Cell proliferation in the gastric epithelium of the ulcer rat. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY 2005; 40(-12-):1386-1393. >>> Thymidine; gastrin-17, synthetic rat; Saline; BSA; SC; Rat; 2ML1; 2ML2; 1, 2, 6, 13 days; Controls received mp w/ agent and no induced ulcer; functionality of mp verified by plasma gastrin levels; multiple pumps per animal (2); animal info (female, Sprague-Dawley, 200-250 g); therapeutic treatment (gastric ulcer); mp primed overnight in 37C saline.

P7500 Lotinun,S., Sibonga,J.D., Turner,R.T. Evidence that the cells responsible for marrow fibrosis in a rat model for hyperparathyroidism are preosteoblasts. Endocrinology 2005; 146(-9-):4074-4081. >>> Parathyroid hormone, human 1-34; thymidine, methyl -3H; Sodium chloride; HCl; serum, rat; ethanol; SC; Rat; 1, 2, 4 weeks; 1, 3, 5 days; Controls received mp w/ vehicle; functionality of mp verified by serum PTH; pumps replaced after 2 weeks; stability verified; peptides; multiple pumps per animal (2); animal info (female, Sprague-Dawley, 260 g); 2% ethanol.

P7340 Thompson,C.K., Brenowitz,E.A., Thompson,C.K. Seasonal change in neuron size and spacing but not neuronal recruitment in a basal ganglia nucleus in the avian song control system. Journal of Comparative Neurology 2005; 481(--):276-283. >>> Thymidine, [3H]; SC; Bird (song sparrow); 1007D; 7 days.

P6496 Helander,H.F., Poorkhalkali,N. Parietal cell density during gastric ulcer healing in the rat. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY 2004; 39(-1-):20-26. >>> Thymidine, (methyl-3H)-; gastrin-17, synthetic rat; Saline; BSA; SC; Rat; 2ML1; 2ML2; 1,2,6,13 days; Functionality of mp verified by gastrin plasma concentrations; multiple pumps per animal (2); mp primed overnight in saline @ 37 degrees Celsius.

P7703 Van Miller,J.P., Garman,R.H., Hermansky,S.J., Mirsalis,J.C., Frederick,C.B. Skin irritation, basal epithelial cell proliferation, and carcinogenicity evaluations of a representative specialty acrylate and methacrylate. Regul.Toxicol.Pharmacol. 2003/2; 37(-1-):54-65. >>> Uridine, bromodeoxy; thymidine, 3H methyl-; PBS, dulbecco's; IP; Mice; 1003D; 2001D; 24 hours; Animal info (male wistar).

P5684 Chouaf-Lakhdar,L., Fevre-Montange,M., Brisson,C., Strazielle,N., Gamrani,H., Didier-Bazes,M. Proliferative activity and nestin expression in periventricular cells of the adult rat brain. NeuroReport 2003; 14(-4-):633-636. >>> Uridine, bromodeoxy-; thymidine; Saline; NaOH (sodium hydroxide); Radio-Isotopes; 3H tracer; CSF/CNS; Rat; 1003D; 3 days; Brain tissue distribution.

P5644 Poorkhalkali,N., Helander,H.F. Cell kinetics of the oesophageal epithelium in the rat: Effects of hypergastrinaemia. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY 2003; 38(-1-):14-19. >>> Thymidine; 3H Tracer; Radio-isotopes; IP; Bird (chicken); 2001; 24 hrs; 6,9,15,17 days; Pumps replaced after 7 days.

P4369 Doetsch,F., Caille,I., Lim,D.A., Garcia-Verdugo,J.M., Alvarez-Buylla,A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell 1999/6/11; 97(--):703-716. >>> Thymidine, [3H]-; Ara-C; Radio-isotopes; Saline; 3H Tracer; CSF/CNS; CSF/CNS (surface of brain); mice; 2002; 1007D; 6, 12 days; Ara-C is cytosine-b-D-arabinofuranoside; controls received mp with vehicle.

P4127 Kuntz,A.L., Oesterle,E.C. Transforming growth factor-a with insulin induces proliferation in rat utricular extrasensory epithelia. Otolaryngol Head Neck Surg 1998; 118(--):816-824. >>> Thymidine; Transforming growth factor-a; Insulin; 3H tracer; Radio-isotopes; ear (cochlea); rat; 1003D; 2001; 3,7 days; controls received mp w/vehicle; tissue perfusion (cochlea); agents infused singly or concomitantly in same pump; ALZET brain infusion kit 1 was modified for cochlear infusion; peptides.

P4099 Jansen,C., Ihse,I., Axelson,J. Epidermal growth factor induces increased mucosal thickness of the small intestine in mouse. Eur.Surg.Res. 1997; 29(--):447-454. >>> Epidermal growth factor; Thymidine; 3H tracer; Radio-isotopes; SC; IP; mice; 1003D; 2001; 1, 3, 7 days; peptides; recomb. human EGF used.

P3552 Ohlsson,B., Jansen,C., Ihse,I., Axelson,J. Epidermal growth factor induces cell proliferation in mouse pancreas and salivary glands. Pancreas 1997; 14(-1-):94-98. >>> Epidermal growth factor; Thymidine; 3H tracer; Radio-isotopes; SC; IP; mice; 2001; 1003D; 1, 3, 7 days; controls received no treatment; peptides; human recomb. EGF used; EGF&[3H]-thymidine given concomitantly.

P3860 Zhu,J., Zhang,L., Hanisch,U.K., Felgner,P.L., Reszka,R. A continuous intracerebral gene delivery system for in vivo liposome-mediated gene therapy. Gene Therapy 1996; 3(--):472-476. >>> Liposomes; Gene, herpes simplex virus thymidine kinase; Gene, lacZ; CSF/CNS (caudate nucleus); Rat; 1003D; 3 days; controls received mp w/LacZ gene; tissue perfusion (tumor); functionality of mp verified by gene expression; comparison of intracerebral injections vs. mp; no stress (see pg.473); stability verified by gene expression; ALZET brain infusion kit used; cancer; gene therapy; "DNA-liposome complexes were stable within minipumps at body temperature (37C) for 1-3 days." (pg.474); "continuous administration of DNA-liposome complexes did not result in significant in vivo toxicity." (pg.474).

P3505 Roberson,D.W., Kreig,C.S., Rubel,E.W. Light microscopic evidence that direct transdifferentiation gives rise to new hair cells in regenerating avian auditory epithelium. Auditory Neuroscience 1996; 2(--):195-205. >>> Thymidine; Radio-isotopes; 3H tracer; ear (cochlea); bird (chicken); 2002; 5,12 days; 26,30 hours; tissue perfusion (cochlea); ALZET brain infusion kit used.

P3380 Kinsella,T.J., Glennon,M.C., Kunugi,K.A., Linstrom,M.J. In vivo modulation of iododeoxyuridine metabolism and incorporation into cellular DNA by 5'-amino-5'-deoxythymidine in normal mouse tissues and two human colon cancer xenografts. Clin.Cancer Res. 1996; 2(--):981-989. >>> Uridine, iododeoxy-; Thymidine, 5'-amino-5'-deoxy-; NaOH; SC; mice (nude); 2001; 6 days; controls received mp w/NaOH; 6 day stability verified by uv spectrophotometry p.983; cancer; toxicology; 1-2 mps used per animal; agents given singly&together.

P3329 Monticello,T.M., Swenberg,J.A., Gross,E.A., Leininger,J.R., Kimbell,J.S., Seilkop,S., Starr,T.B., Gibson,J.E., Morgan,K.T. Correlation of regional and nonlinear formaldehyde-induced nasal cancer with proliferating populations of cells. Cancer Res. 1996; 56(--):1012-1022. >>> Thymidine, methyl; 3H tracer; Radio-isotopes; SC; rat; 2ML1; 5 days; cancer.

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