ALZET® Osmotic Pump References: The Rate-Controlled Modulation of Hormone Levels

This collection of references illustrates techniques by which the physiological effects of a controlled hormone infusion are determined in the absence of endogenous hormone production. In this manner, the effects that hormones have at different concentrations can be determined independent of temporal fluctuations in hormone levels. The technical notes following each reference detail the substance(s) infused, the route of administration, the animal model studied, the vehicle for infusion, the model of pump used, the duration of infusion, and notable technical achievements or results obtained.
This list does not contain references in this category from before 1995.

To obtain a complete list of citations pertaining to extended duration studies published since 1975, please contact ALZET Technical Services at (800) 692-2990 (U.S. & Canada), or (408) 367-4036. You may also contact us via e-mail at alzet@durect.com.

The inverse of this technique, or the study of the differential effects of varying regimens of pulsatile hormone administration, can be accessed through the sub-bibliography entitled "References on Pulsed Administration of Agents by ALZET Osmotic Pumps". To obtain a copy of this sub-bibliography call ALZET Technical Services at the numbers listed above.

For a more complete discussion of the capabilities of these techniques consult the following reference:
Urquhart, J., Fara, J., and Willis, K.L. (1984). Rate-controlled delivery systems in drug and hormone research. Ann. Rev. Pharmacol. Toxicol. 24, 199-236.

R0241 Crowley,W.R., Ramoz,G., Torto,R., Keefe,K.A., Wang,J.J., Kalra,S.P. Neuroendocrine actions and regulation of hypothalamic neuropeptide Y during lactation. Peptides 2007; 28(-2-):447-452. >>> Leptin, recomb. rat; Insulin; SC; Rat; ; Replacement therapy (suckling induced hypoleptinemia); peptides; animal info (female, lactating); see p. 450; endocrinology.

P8379 Thippeswamy,T., Haddley,K., Corness,J.D., Howard,M.R., McKay,J.S., Beaucourt,S.M., Pope,M.D., Murphy,D., Morris,R., Hoekfelt,T., Quinn,J.P. NO-cGMP mediated galanin expression in NGF-deprived or axotomized sensory neurons. Journal of Neurochemistry 2007; 100(-3-):790-801. >>> Nitroindazole, 7-; DMSO; IP; Rat; 2ML1; 7 days; Controls received mp w/ vehicle; replacement therapy (axotomy); animal info (male, Wistar, Albino, 250-280g.); 20% DMSO.

P8365 Diniz,G.P., Carneiro-Ramos,M.S., Barreto-Chaves,M.L.M. Angiotensin type 1 (AT1) and type 2 (AT2) receptors mediate the increase in TGF-b1 in thyroid hormone-induced cardiac hypertrophy. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 2007; 454(-1-):75-81. >>> PD123319; SC; Rat; 15 days; Replacement Therapy (T4-induced hyperthroidism; cardiovascular; animal info (Wistar, 220-250g.).

P8361 Gangula,P.R.R., Maner,W.L., Micci,M.A., Garfield,R.E., Pasricha,P.J. Diabetes induces sex-dependent changes in neuronal nitric oxide synthase dimerization and function in the rat gastric antrum. American Journal of Physiology-Gastrointestinal and Liver Physiology 2007; 292(-3-):G725-G733. >>> L-NAME; SC; Rat; 2ML1; 4 days; Replacement therapy (STZ-induced diabetes mellitus; enzyme inhibitor (Nitric oxide synthase); animal info (male, female, Sprague-Dawley, 7 wks old).

P8334 Yao,Z.L., Zhao,B.T., Hoffman,E.P., Ghimbovschi,S., DuBois,D.C., Almon,R.R., Jusko,W.J. Application of scaling factors in simultaneous modeling of microarray data from diverse chips. PHARMACEUTICAL RESEARCH 2007; 24(-4-):643-649. >>> Methylprednisdone; Saline; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy); comparison of IV bolus injection vs. mp; animal info (male, Wistar).

P8310 Singh,A.K., Gudehithlu,K.P., Litbarg,N.O., Sethupathi,P., Arruda,J.A.L., Dunea,G. Transplanting fragments of diabetic pancreas into activated omentum gives rise to new insulin producing cells. Biochemical and Biophysical Research Communications 2007; 355(-1-):258-262. >>> Insulin; SC; Rat; 28 days; Replacement therapy (STZ-induced diabetes, pancreatectomy and autotransplanted into omentum; immunology; animal info (male, Sprague-Dawley, 150-200g.); nephrology.

P8299 Cruze,C.A., Su,F., Limberg,B.J., Deutsch,A.J., Stoffolano,P.J., Dai,H.J., Buchanan,D.D., Yang,H.T., Terjung,R.L., Spruell,R.D., Mittelstadt,S.W., Rosenbaum,J.S. The Y2 receptor mediates increases in collateral-dependent blood flow in a model of peripheral arterial insufficiency. Peptides 2007; 28(-2-):269-280. >>> Peptide YY, recomb. human; peptide YY (3-36), recomb. human; vascular endothelial growth factor 165; PBS; glycerol; sodium citrate; tween 20; IA (iliac); Rat; 14 days; Controls received mp w/ vehicle; replacement therapy (femoral artery occlusion); dose-response (fig. 4); half-lfie (p. 276)<30 minutes; cardiovascular; peptides; ischemia (hindlimb); animal info (male, Sprague-Dawley, 325-350 grams).

P8263 Nishimura,M., Takahashi,H., Yoshimura,M. Upregulation of the brain renin-angiotensin system in rats with chronic renal failure. ACTA PHYSIOLOGICA 2007; 189(-4-):369-377. >>> Candesartan; CSF, artificial; IV (jugular); CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle; replacement therapy (subtotal nephrectomy, dorsal rhizotomy); antihypertensive; cyanoacrylate adhesive; animal info (male, SHR/Izumo, 8 wks old, 300-350g.); ATI receptor antagonist.

P8255 Li,J.H., Deane,J.A., Campanale,N.V., Bertram,J.F., Ricardo,S.D. The contribution of bone marrow-derived cells to the development of renal interstitial fibrosis. Stem Cells 2007; 25(-3-):697-706. >>> SB203580; alk5; Saline; Mice; 2, 4 weeks; Controls received mp w/ vehicle; replacement therapy (ADR-induced nephropathy); dose-response (pg. 698); enzyme inhibitor (pg. 38 MAP kinase); animal info (male, BALB/C, bone marrow transplant, 20 wks old); ADR is adriamycin, TGF-B receptor I (ALK5) inhibitor.

P8167 Bidani,A.K., Picken,M., Hacioglu,R., Williamson,G., Griffin,K.A. Spontaneously reduced blood pressure load in the rat streptozotocin-induced diabetes model: potential pathogenetic relevance. American Journal of Physiology-Renal Physiology 2007; 292(-2-):F647-F654. >>> Insulin; SC; Rat; 16-20, 36-40 weeks; Replacement therapy (STZ-induced Diabetes); long-term study; pumps replaced every 4 weeks; peptides; animal info (male, Sprague-Dawley, 8 weeks old, 250 grams).

P8151 Hirono,Y., Yoshimoto,T., Suzuki,N., Sugiyama,T., Sakurada,M., Takai,S., Kobayashi,N., Shichiri,M., Hirata,Y. Angiotensin II receptor type 1-mediated vascular oxidative stress and proinflammatory gene expression in aldosterone-induced hypertension: The possible role of local renin-angiotensin system. Endocrinology 2007; 148(-4-):1688-1696. >>> Aldosterone, D-; Water, distilled; propylene glycol; ethanol; SC; Rat; 2004; 3 weeks; Blood pressure taken; replacement therapy (uninephrectomy); cardiovascular; animal info (7 weeks old, male, Sprague-Dawley); 9% ethanol used.

P8149 Kovacs,P., Morales,J.C., Karkanias,G.B. The effect of diabetes and centrally administered insulin on anterior hypothalamic estrogen receptor alpha immunoreactivity. ACTA DIABETOLOGICA 2007; 44(-1-):38-44. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (STZ-induced Diabetes, ovariectomy); peptides; animal info (female, Sprague-Dawley, 175-200g.).

P8128 Hartner,A., Cordasic,N., Klanke,B., Wittmann,M., Veelken,R., Hilgers,K.F. Renal injury in streptozotocin-diabetic Ren2-transgenic rats is mainly dependent on hypertension, not on diabetes. American Journal of Physiology-Renal Physiology 2007; 292(-2-):F820-F827. >>> Losartan; IP; Rat; rat (transgenic); 2004; 4 weeks; Controls received mp w/ saline; replacement therapy (STZ-induced diabetes mellitus); animal info (male, Sprague-Dawley-Hanover, ren-2 Tg, 12 wks old, 250g.).

P8066 Bielinska,M., Seehra,A., Toppari,J., Heikinheimo,M., Wilson,D.B. GATA-4 is required for sex steroidogenic cell development in the fetal mouse. Developmental Dynamics 2007; 236(-1-):203-213. >>> Luteinizing hormone releasing hormone, [D-Trp6]-; SC; Mice (nude); 3 weeks; Replacement therapy (gonadectomy); peptides; animal info (female, NU/J, chimeric teratoma).

P7976 Hewitson,T.D., Mookerjee,I., Masterson,R., Zhao,C.X., Tregear,G.W., Becker,G.J., Samuel,C.S. Endogenous relaxin is a naturally occurring modulator of experimental renal tubulointerstitial fibrosis. Endocrinology 2007; 148(-2-):660-669. >>> Relaxin; Sodium acetate; SC; Mice; 1007D; ; Replacement therapy (RLX -/-); peptides; animal info (4-6 weeks old).

P8358 Klein,J.D., Kozlowski,S., Antoun,T.A., Sands,J.M. Adrenalectomy blocks the compensatory increases in UT-A1 and AQP2 in diabetic rat kidney. Journal of Membrane Biology 2006; 212(-2-):139-144. >>> Aldosterone; Rat; 14 days; Replacement therapy (adrenalectomy, STZ-induced diabetes mellitus); animal info (male, Sprague-Dawley, 100-150 grams); endocrinology.

P8355 rias-Loza,P.A., Hu,K., Schaefer,A., Bauersachs,J., Quaschning,T., Galle,J., Jazbutyte,V., Neyses,L., Ertl,G., Fritzemeier,K.H., Hegele-Hartung,C., Pelzer,T. Medroxyprogesterone acetate but not drospirenone ablates the protective function of 17 beta-estradiol in aldosterone salt-treated rats. Hypertension 2006; 48(-5-):994-1001. >>> Aldosterone; SC; Rat; ; Controls received sham treatment; replacement therapy (ovariectomy, uninephrectomy); cardiovascular; animal info (female, Wistar, 250-300 grams).

P8317 Omata,M., Taniguchi,H., Koya,D., Kanasaki,K., Sho,R., Kato,Y., Kojima,R., Haneda,M., Inomata,N. N-acetyl-seryl-aspartyl-lysyl-proline ameliorates the progression of renal dysfunction and fibrosis in WKY rats with established anti-glomerular basement membrane nephiritis. Journal of the American Society of Nephrology 2006; 17(-3-):674-685. >>> Peptide, Ac-SDKP; Saline; acetic acid; SC; Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma Ac-SDKP levels; replacement therapy (anti-GBM Nephritis); peptides; animal info (male, Wistar-Kyoto, 7 wks old).

P8229 Chen,P., Scicli,G.M., Guo,M., Fenstermacher,J.D., Dahl,D., Edwards,P.A., Scicli,A.G. Role of angiotensin II in retinal leukostasis in the diabetic rat. Experimental Eye Research 2006; 83(-5-):1041-1051. >>> Losartan; IP; Rat; 2 weeks; Controls received no treatment; replacement therapy (STZ-induced diabetes); animal info (male, Long-Evans, 200-225 grams); Ang II-AT1 receptor antagonist.

P8217 Castellano,J.M., Navarro,V.M., Fernandez-Fernandez,R., Roa,J., Vigo,E., Pineda,R., Dieguez,C., Aguilar,E., Pinilla,L., Tena-Sempere,M. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 2006; 55(-9-):2602-2610. >>> Insulin; leptin; Saline; HCI; CSF/CNS; Rat; 2001; 6 days; Controls received mp w/ vehicle, or nondiabetic; replacement therapy (STZ-induced Diabetes); peptides; animal info (male, Wistar, adult).

P8140 Husen,B., Huhtinen,K., Saloniemi,T., Messinger,J., Thole,H.H., Poutanen,M. Human hydroxysteroid (17-beta) dehydrogenase 1 expression enhances estrogen sensitivity of MCF-7 breast cancer cell xenografts. Endocrinology 2006; 147(-11-):5333-5339. >>> B10721325; estrone; DMSO; propanediol; SC; Mice (nude); 1002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); dose-response (p.5335); pumps replaced after 14 days; enzyme inhibitor (hydroxysteroid 17 beta dehydrogenase 1); cancer (breast); animal info (female, BALB/cA Bom-v, Crl: nu/nu, 10-11 wks old); 50%DMSO; endocrinology.

P8139 Huang,Y., Border,W.A., Lawerence,D.A., Noble,N.A. Noninhibitory PAI-1 enhances plasmin-mediated matrix degradation both in vitro and in experimental nephritis. Kidney International 2006; 70(-3-):515-522. >>> Tranexamic acid; SC; Rat; 2ML1; 4 days; Replacement therapy (glomerulonephritis); dose-response (p.521); enzyme inhibitor (plasmin/plasminogen); animal info (male, Sprague-Dawley, 18-200g.).

P8112 Ji,B., Li,M., Wu,W.T., Yick,L.W., Lee,X., Shao,Z., Wang,J., So,K.F., Mccoy,J.M., Pepinsky,R.B., Mi,S., Relton,J.K. LINGO-1 antagonist promotes functional recovery and axonal sprouting after spinal cord injury. MOLECULAR AND CELLULAR NEUROSCIENCE 2006; 33(-3-):311-320. >>> Immunoglobulin G1, human; LINGO-1-Fc, recomb. human; PBS; CSF/CNS (intrathecal, subarachnoid space); Rat; 2004; 4 weeks; Controls received mp w/ vehicle, or IgG1; replacement therapy (dorsal hemisection); peptides; post op. care (Buprenorphine/Buprenex, Ampicillin); animal info (female, Long Evans, 7 wk old).

P8100 Nehme,J., Mercier,N., Labat,C., Benetos,A., Safar,M.E., Delcayre,C., Lacolley,P. Differences between cardiac and arterial fibrosis and stiffness in aldosterone-salt rats: Effect of eplerenone. JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM 2006; 7(-1-):31-39. >>> Aldosterone, D-; SC; Rat; 2004; 28 days; Controls received mp w/ physiological serum; replacement therapy (uninephrectomy); cardiovascular; animal info (male, Sprague-Dawley, 8 wk old, 300g.).

P8088 Fulton,S., Pissios,P., Manchon,R.P., Stiles,L., Frank,L., Pothos,E.N., Maratos-Flier,E., Flier,J.S. Leptin regulation of the mesoaccumbens dopamine pathway. Neuron 2006; 51(-6-):811-822. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1003D; 1002; 3, 11, 14 days; Controls received mp w/ vehicle; replacement therapy (ob/ob mice); peptides; animal info (male, C57BL/6J wt, ob/ob or Lepr (db/db); 4-10 wks old); mp primed 4-6 hrs. at 37 celcius.

P7993 Vandenberg,L.N., Wadia,P.R., Schaeberle,C.M., Rubin,B.S., Sonnenschein,C., Soto,A.M. The mammary gland response to estradiol: Monotonic at the cellular level, non-monotonic at the tissue-level of organization?. Journal of Steroid Biochemistry and Molecular Biology 2006; 101(-4-5-):263-274. >>> Estradiol, 17 beta; DMSO; SC; Mice; 1002; 10 days; Controls received mp w/ vehicle; replacement therapy (OVX); dose-response (267); animal info (female, CD-1, OVX); 50% DMSO used.

P7985 Warne,J.P., Horneman,H.F., Wick,E.C., Bhargava,A., Pecoraro,N.C., Ginsberg,A.B., Akana,S.F., Dallman,M.F. Comparison of superior mesenteric versus jugular venous infusions of insulin in streptozotocin-diabetic rats on the choice of caloric intake, body weight, and fat stores. Endocrinology 2006; 147(-11-):5443-5451. >>> Insulin, methylene blue; Saline; IV (jugular); Rat; 2002; ; Functionality of mp verified by dye infusion; replacement therapy (diabetic); no stress (see pg. 5444); good methods; peptides; animal info (male, Sprague-Dawley, 275 grams); diabetes; PE-60 fused to PE-5.

P7944 Shao,T.C., Li,H.L., Kasper,S., Matusik,R., Ittmann,M., Cunningham,G.R. Comparison of the growth-promoting effects of testosterone and 7-alpha-methyl-19-nor-testosterone (MENT) on the prostate and levator ani muscle of LPB-Tag transgenic mice. PROSTATE 2006; 66(-4-):369-376. >>> Testosterone; nortestosterone, 7 alpha-methyl-19-; Cyclodextrin; SC; Mice (transgenic); 2004; 4 weeks; Replacement therapy (orchidectomy); cancer (prostate); animal info (male, transgenic, 32 grams, ORX); MENT (7-alpha-methyl-19-nor-testosterone) and testosterone dissolved in 45% cyclodextrin.

P7927 Tiwari,S., Packer,R.K., Hu,X.Q., Sugimura,Y., Verbalis,J.G., Ecelbarger,C.A. Increased renal alpha-ENaC and NCC abundance and elevated blood pressure are independent of hyperaldosteronism in vasopressin escape. American Journal of Physiology-Renal Physiology 2006; 291(-1-):F49-F57. >>> Vasopressin, 1-desamino-8-D-arginine; aldosterone; SC; Rat; 2002; ; Blood pressure taken; replacement therapy (adrenalectomy); multiple pumps per animal (2); animal info (male, Sprague-Dawley, 275 grams, ADX); dDAVP; one pump for each drug.

P7902 Husen,B., Huhtinen,K., Poutanen,M., Kangas,L., Messinger,J., Thole,H. Evaluation of inhibitors for 17 beta-hydroxysteroid dehydrogenase type 1 in vivo in immunodeficient mice inoculated with MCF-7 cells stably expressing the recombinant human enzyme. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2006; 248(-1-2-):109-113. >>> Estrone; B10720512; B10721325; B10720440; B10720511; B10715817; DMSO; propanediol; SC; Mice (nude); 1002; 4 weeks; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); dose-response (fig. 5); pumps replaced after 2 weeks; enzyme inhibitor (17 beta-hydroxysteroid dehydrogenase); cancer (breast carcinoma); animal info (female, Crl: NU/NU, 10-11 wk old); 50% DMSO.

P7896 Diano,S., Farr,S.A., Benoit,S.C., McNay,E.C., da Silva,I., Horvath,B., Gaskin,F.S., Nonaka,N., Jaeger,L.B., Banks,W.A., Morley,J.E., Pinto,S., Sherwin,R.S., Xu,L., Yamada,K.A., Sleeman,M.W., Tschoep,M.H., Horvath,T.L. Ghrelin controls hippocampal spine synapse density and memory performance. Nature Neuroscience 2006; 9(-3-):381-388. >>> Ghrelin; Saline; SC; Mice; 14 days; Controls received mp w/ vehicle; replacement therapy (ghrelin knockout); peptides; animal info (C57/B16, Ghsr -/-, male); neurodegenerative (Alzheimer's disease).

P7879 Lu,H., Klaassen,C. Tissue distribution and thyroid hormone regulation of Pept1 and Pept2 mRNA in rodents. Peptides 2006; 27(-4-):850-857. >>> Triiodothyromine; thyroxine, L-; Saline; NaOH; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle, or no treatment; replacement therapy (thyroidectomy); animal info (Sprague-Dawley, 5 wk old, male).

P7877 Kaneko,H., Kikuchi,K., Noguchi,J., Ozawa,M., Ohnuma,K., Maedomari,N., Kashiwazaki,N. Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice. REPRODUCTION 2006; 131(-2-):279-288. >>> Follicle-stimulating hormone, porcine; Saline; SC; Mice (nude); 2004; 7, 14 days; Controls received no hormone treatment; replacement therapy (ovariectomy); dose-response (pg. 280); animal info (female, Crlj: CD1-Foxn1nu, 5-6 wk old); ovarian xenografts.

P7876 Julien,C., Berthiaume,L., Hadj-Tahar,A., Rajput,A.H., Bedard,P.J., Di Paolo,T., Julien,P., Calon,F. Postmortem brain fatty acid profile of levodopa-treated Parkinson disease patients and parkinsonian monkeys. NEUROCHEMISTRY INTERNATIONAL 2006; 48(-5-):404-414. >>> MPTP; Water, sterile; SC; Monkey; 4 weeks; Controls received no treatment; replacement therapy (ovariectomy); neurodegenerative (Parkinson's disease); animal info (female, cynomolgus, 3.0-4.3 kg.).

P7832 Yamada,M., Shibusawa,N., Ishii,S., Horiguchi,K., Umezawa,R., Hashimoto,K., Monden,T., Satoh,T., Hirato,J., Mori,M. Prolactin secretion in mice with thyrotropin-releasing hormone deficiency. Endocrinology 2006; 147(-5-):2591-2596. >>> Thyrotropin-releasing hormone; SC; Mice; 2002; 14 days; Replacement therapy (TRH -/-); peptides; animal info (female, lactating, TRH -/-).

P7831 Windle,R.J., Gamble,L.E., Kershaw,Y.M., Wood,S.A., Lightman,S.L., Ingram,C.D. Gonadal steroid modulation of stress-induced hypothalamo-pituitary-adrenal activity and anxiety behavior: Role of central oxytocin. Endocrinology 2006; 147(-5-):2423-2431. >>> Oxytocin antagonist; oxytocin antagonist, desGly-; CSF/CNS; Rat; 1007D; 5 days; Controls received mp w/ saline; replacement therapy (ovariectomy); ALZET brain infusion kit used; peptides; animal info (female, Sprague-Dawley, 225-250g.); mp primed overnight in isotonic saline.

P7829 Toth,C., Brussee,V., Zochodne,D.W. Remote neurotrophic support of epidermal nerve fibres in experimental diabetes. Diabetologia 2006; 49(-5-):1081-1088. >>> Insulin; insulin-like growth factor-1; Saline; SC; CSF/CNS (intrathecal, lumbar); Rat; 2002; 1 month; Controls received mp w/ vehicle; replacement therapy (stz-induced diabetes); pumps replaced after 2 weeks; peptides; animal info (male, Sprague-Dawley, 300-350g.); comparison of SC mp vs. IT mp.

P7809 Ma,J., Weisberg,A., Griffin,J.P., Vaughan,D.E., Fogo,A.B., Brown,N.J. Plasminogen activator inhibitor-1 deficiency protects against aldosterone- induced glomerular injury. Kidney International 2006; 69(-6-):1064-1072. >>> Aldosterone, D-; DMSO; SC; Mice; 2004; 12 weeks; Controls received mp w/ vehicle; replacement therapy (uninephrectomy); long-term study; pumps replaced every 4 weeks; cardiovascular; animal info (male, C57BL/6J wt or PAI-1(-/-), 8-10 wk old, 27g.); 3.6% DMSO; nephrology.

P7793 Seto,S., Nagao,S., Ozeki,S., Tetsuo,H., Akahoshi,M., Yano,K. Contribution of central nitric oxide to the regulation of blood pressure and sodium balance in DOCA-salt hypertension. Journal of Cardiovascular Pharmacology 2006; 47(-5-):680-685. >>> L-NMMA; IP; CSF/CNS; Rat; 2ML4; 4 weeks; Controls received mp w/ saline; replacement therapy (nephrectomy); dose-response (fig. 1); enzyme inhibitor (no synthase); cardiovascular; animal info (male, Wistar, 7 wk. old, 240g.); cannula location confirmed by methylene blue staining.

P7777 Fan,Y.Y., Baba,R., Nagai,Y., Miyatake,A., Hosomi,N., Kimura,S., Sun,G.P., Kohno,M., Fujita,M., Abe,Y., Nishiyama,A. Augmentation of intrarenal angiotensin II levels in uninephrectomized aldosterone/salt-treated hypertensive rats; Renoprotective effects of an ultrahigh dose of olmesartan. HYPERTENSION RESEARCH 2006; 29(-3-):169-178. >>> Aldosterone; Ethanol; SC; Rat; 2002; 3, 6 weeks; Controls received mp w/ vehicle; replacement therapy (uninephrectomy); long-term study; pumps replaced every 2 weeks; cardiovascular; animal info (male, Sprague-Dawley, 162-184g.); 2% ETOH.

P7773 Yang,Z.D., Chen,M., Carter,J.D., Nunemaker,C.S., Garmey,J.C., Kimble,S.D., Nadler,J.L. Combined treatment with lisofylline and exendin-4 reverses autoimmune diabetes. Biochemical and Biophysical Research Communications 2006; 344(-3-):1017-1022. >>> Lisofylline; exendin-4; Saline; SC; Mice; 2004; 4 weeks; Controls received mp w/ vehicle; replacement therapy (non-obese diabetes); animal info (C57BL/6J, NOD/LtJ, 18-30 wk. old).

P7760 Waeckel,L., Bignon,J., Liu,J.M., Markovits,D., Ebrahimian,T.G., Vilar,J., Mees,B., Blanc-Brude,O., Barateau,V., Le ricousse-Roussanne,S., Duriez,M., Tobelem,G., Wdzieczak-Bakala,J., Levy,B.I., Silvestre,J.S. Tetrapeptide AcSDKP induces postischemic neovascularization through monocyte chemoattractant protein-1 signaling. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 2006; 26(-4-):773-779. >>> Peptide, AcSKDP; monocyte chemoattractant protein-1; SC; Mice; 2001; 2004; 7, 21 days; Controls received mp w/ saline; replacement therapy (MCP-1 deficient); dose-response (fig. 1); cardiovascular; peptides; multiple pumps per animal (2); ischemia (hind limb); animal info (c57BI/6, wt or MCP-1 deficient, 10 wk old).

P7744 Simon,F.R., Iwahashi,M., Hu,L.J., Qadri,I., Arias,I.M., Ortiz,D., Dahl,R., Sutherland,E. Hormonal regulation of hepatic multidrug resistance-associated protein 2 (Abcc2) primarily involves the pattern of growth hormone secretion. American Journal of Physiology-Gastrointestinal and Liver Physiology 2006; 290(-4-):G595-G608. >>> Growth hormone; Sodium phosphate buffer; NaN2; glycerol; SC; Rat; mice; 2001; 7 days; Controls received sham surgery; replacement therapy (hypophysectomy); comparison of SC injections vs. mp; peptides; animal info (adult, Sprague-Dawley, male, female, 170-225g.).

P7743 Wang,W.D., Li,C.L., Summer,S.N., Falk,S., Cadnapaphornchai,M.A., Chen,Y.C., Schrier,R.W. Molecular analysis of impaired urinary diluting capacity in glucocorticoid deficiency. American Journal of Physiology-Renal Physiology 2006; 290(-5-):F1135-F1142. >>> Aldosterone; IP; Rat; 2ML4; 12 days; Replacement therapy (adrenalectomy); animal info (male, Wistar, 160-180g.).

P7667 Geerling,J.C., Engeland,W.C., Kawata,M., Loewy,A.D. Aldosterone target neurons in the nucleus tractus solitarius drive sodium appetite. Journal of Neuroscience 2006; 26(-2-):411-417. >>> Aldosterone; Saline; ethanol; IP; Rat; 2ML1; 7 days; Controls received mp w/ vehicle; replacement therapy (bilateral adrenalectomy); animal info (male, Sprague-Dawley, 275-325 g); 1 % ETOH.

P7627 Ruan,W.F., Fahlbusch,F., Clemmons,D.R., Monaco,M.E., Walden,P.D., Silva,A.P., Schmid,H.A., Kleinberg,D.L. SOM230 inhibits insulin-like growth factor-I action in mammary gland development by pituitary independent mechanism: Mediated through somatostatin subtype receptor 3?. MOLECULAR ENDOCRINOLOGY 2006; 20(-2-):426-436. >>> SOM230; octreotide; SC; Rat; 2001; 5, 7 days; Controls received mp w/ vehicle; replacement therapy (hypophsectomy, ophorectomy); animal info (female, CD, 21 days old); somatostatin analog.

P7573 Mark,P.J., Smith,J.T., Waddell,B.J. Placental and fetal growth retardation following partial progesterone withdrawal in rat pregnancy. PLACENTA 2006; 27(-2-3-):208-214. >>> Estradiol; Propylene glycol; SC; Rat (pregnant); 6 days; Controls received sham operation and 2x daily injection of vehicle; functionality of mp verified by plasma estradiol concentrations; replacement therapy (ovariectomy); animal info (female, albino Wistar, 3-5 month old).

P7538 Jain,R.N., Brunkan,C.S., Chew,C.S., Samuelson,L.C. Gene expression profiling of gastrin target genes in parietal cells. PHYSIOLOGICAL GENOMICS 2006; 24(-2-):124-132. >>> Gastrin, rat; Saline; BSA; SC; Mice; 2001; 7 days; Controls received mp w/ vehicle; replacement therapy (gastrin knockout); animal info (GAS-KO, male, 8-10 wk old); mp primed overnight in saline.

P7513 Vidal,A., Sun,Y., Bhattacharya,S.K., Ahokas,R.A., Gerling,I.C., Weber,K.T. Calcium paradox of aldosteronism and the role of the parathyroid glands. American Journal of Physiology-Heart and Circulatory Physiology 2006; 290(-1-):H286-H294. >>> Aldosterone; Rat; 1, 2, 4, 6 weeks; Controls received no treatment; replacement therapy (uninephrectomy, parathyroidectomy); cardiovascular; animal info (male, Sprague-Dawley, 9 wk old).

P7503 Shearman,L.P., Wang,S.P., Helmling,S., Stribling,D.S., Mazur,P., Ge,L., Wang,L.Y., Klussmann,S., MacIntyre,D.E., Howard,A.D., Strack,A.M. Ghrelin neutralization by a ribonucleic acid-SPM ameliorates obesity in diet-induced obese mice. Endocrinology 2006; 147(-3-):1517-1526. >>> Antisense, NOX-B11-2; RNA, spiegelmer L-; MT-II; PBS; SC; Mice; 2002; 7, 14 days; Controls received mp w/ vehicle or control SPM RNA; replacement therapy (diet-induced obesity); half-life (p.1525) 10-14 hours; animal info (male, C57BL/6N, 47 g, ghrelin -/-, WT, 41 g); synthetic RNA spiegelmer (SPM) oli.

P8063 Chen,Y.C., Cadnapaphornchai,M.A., Summer,S.N., Falk,S., Li,C.L., Wang,W.D., Schrier,R.W. Molecular mechanisms of impaired urinary concentrating ability in glucocorticoid-deficient rats. Journal of the American Society of Nephrology 2005; 16(-10-):2864-2871. >>> Aldosterone; IP; Rat; 2ML4; 11 days; Controls received mp w/ agent plus SC injections of dexamethasone; replacement therapy (adrenalectomy); animal info (male, Sprague-Dawley, 175-200g.); nephrology.

P7756 Shibuya,K., Kanasaki,K., Isono,M., Sato,H., Omata,M., Sugimoto,T., Araki,S.I., Isshiki,K., Kashiwagi,A., Haneda,M., Koya,D. N-acetyl-seryl-aspartyl-lysyl-proline prevents renal insufficiency and mesangial matrix expansion in diabetic db/db mice. Diabetes 2005; 54(-3-):838-845. >>> Peptide, AcSDKP; Balanced salt solution; SC; Mice; 2004; 8 weeks; Controls received mp w/ vehicle, or controld peptide; functionality of mp verified by plasma AC-SDKP levels; replacement therapy (diabetes); long-term study; pumps replaced at 4 weeks; no stress (see pg. 839, 843); peptides; animal info (male, db/db, db/m, 10 wk. old).

P7673 Dragonas,C., Mueller,A., Maltaris,T., Kraemer,P., Dimmler,A., Jaeger,W., Beckmann,M.W., Dittrich,R. Role of insulin in the progression of ovarian sex cord stromal tumors in rats. Journal of Cancer Research and Clinical Oncology 2005; 131(-11-):751-757. >>> Insulin; SC; Rat; 28, 56, 84 days; Controls received mp w/ saline; replacement therapy (ovariectomy, STZ-induced diabetes); long-term study; pumps replaced every 28 days; no stress (see pg.753); cancer (sex cord stomal); animal info (female, Lewis, 60 days old, 150-210 g).

P7672 Gorin,Y., Block,K., Hernandez,J., Bhandari,B., Wagner,B., Barnes,J.L., Abboud,H.E. Nox4 NAD(P) H oxidase mediates hypertrophy and fibronectin expression in the diabetic kidney. Journal of Biological Chemistry 2005; 280(-47-):39616-39626. >>> Oligonucleotide, phosphorothioate antisense; oligonucleotide, phosphorothioate sense; SC; Rat; 2 weeks; Controls received mp w/ sense oligo or not treatment; functionality of mp verified by Nox4 levels in kidney cortex; replacement therapy (STZ-induced diabetes); anti-sense (Nox4); animal info (male, Sprague-Dawley, 200-225 g).

P7668 Hasuike,S., Ido,A., Uto,H., Moriuchi,A., Tahara,Y., Numata,M., Nagata,K., Hori,T., Hayashi,K., Tsubouchi,H. Hepatocyte growth factor accelerates the proliferation of hepatic oval cells and possibly promotes the differentiation in a 2-acetylaminofluorene/partial hepatectomy model in rats. Journal of Gastroenterology and Hepatology 2005; 20(-11-):1753-1761. >>> Hepatocyte growth factor, recomb. human; PBS; IP; Rat; 7 days; Controls received mp w/ vehicle; functionality of mp verified by human HGF serum levels; replacement therapy (hepatectomy); half-life (pg. 1758)<3 min; animal info (male, Fisher, 8 wk. old).

P7660 Knauf,C., Cani,P.D., Perrin,C., Iglesias,M.A., Maury,J.F., Bernard,E., Benhamed,F., Gremeaux,T., Drucker,D.J., Kahn,C.R., Girard,J., Tanti,J.F., Delzenne,N.M., Postic,C., Burcelin,R. Brain glucagon-like peptide-1 increases insulin secretion and muscle insulin resistance to favor hepatic glycogen storage. Journal of Clinical Investigation 2005; 115(-12-):3554-3563. >>> Exendin (9-39); IP; Mice; 4 weeks; Controls received mp w/ saline; replacement therapy (diet-induced diabetes); Alzet brain infusion kit used; animal info (C57BL/6J, male, 12 week old); BIK used with other apparatus for short term icv infusion.

P7644 Wang,X.X., Wu,J., Chiba,H., Yamada,K., Ishimi,Y. Puerariae radix prevents bone loss in castrated male mice. METABOLISM-CLINICAL AND EXPERIMENTAL 2005; 54(-11-):1536-1541. >>> Estradiol, 17B; SC; Mice; 4 weeks; Controls received sham surgery with no mp; replacement therapy (orchidectomy); animal info (male, ddY, 8 week old).

P7638 Van Abel,M., Hoenderop,J.G.J., van der Kemp,A.W.C.M., Friedlaender,M.M., van Leeuwen,J.P.T.M., Bindels,R.J.M. Coordinated control of renal Ca2+ transport proteins by parathyroid hormone. Kidney International 2005; 68(-4-):1708-1721. >>> Parathyroid hormone, bovine 1-34; NPS R-467; Cystein HCl; cyclodextrin, 2 hydroxypropyl-beta; SC; Rat; mice; 1007D; 2001; 7 days; Controls received mp w/ vehicle; replacement therapy (parathyroidectomy); dose-response (table 3); animal info (C57BL, male, sabra 250 g, 8 wk old).

P7595 Danzi,S., Dubon,P., Klein,I. Effect of serum triiodothyronine on regulation of cardiac gene expression: role of histone acetylation. American Journal of Physiology-Heart and Circulatory Physiology 2005; 289(-4-):H1506-H1511. >>> Triiodothyronine; NaOH; saline; SC; Rat; 1002; 3, 12 days; Controls received mp w/ vehicle or euthyroid; functionality of mp verified by serum T3 levels; replacement therapy (thyroidectomy); dose-response (fig. 1); comparison of IM injections vs. mp; half-life (pg. H1506, H1508) 7 hours in vivo in rat; cardiovascular; animal info (male, Sprague-Dawley 200 g); mp primed 24 hrs in 37 C saline; "constant infusions leads to stable serum levels by 72 h." (p. H1508; "Bolus injection was not sufficient to normalize serum T3" (p. H1510).

P7564 Ichinose,K., Maeshima,Y., Yamamoto,Y., Kitayama,H., Takazawa,Y., Hirokoshi,K., Sugiyama,H., Yamasaki,Y., Eguchi,K., Makino,H. Antiangiogenic endostatin peptide ameliorates renal alterations in the early stage of a type 1 diabetic nephropathy model. Diabetes 2005; 54(-10-):2891-2903. >>> Endostatin, human (1-27); PBS; SC; Mice; 2004; 2 weeks; Controls received mp w/ vehicle; replacement therapy (STZ diabetes); dose-response (fig. 2); no stress (see pg.2892); peptides; animal info (female, C57BL/6, 21-26 g).

P7555 Plank,C., Hartner,A., Klanke,B., Geissler,B., Porst,M., Amann,K., Hilgers,K.F., Rascher,W., Doetsch,J. Adrenomedullin reduces mesangial cell number and glomerular inflammation in experimental mesangioproliferative glomerulonephritis. Kidney International 2005; 68(-3-):1086-1095. >>> Adrenomedullin, recomb. rat; Aqua injectable; SC; Rat; 2001; 3 days; Controls received mp w/ vehicle; replacement therapy (nephritis); no stress (see pg. 1089); animal info (male, Sprague-Dawley).

P7554 Lautrette,A., Li,S.Q., Alili,R., Sunnarborg,S.W., Burtin,M., Lee,D.C., Friedlander,G., Terzi,F. Angiotensin II and EGF receptor cross-talk in chronic kidney diseases: a new therapeutic approach. Nature Medicine 2005; 11(-8-):867-874. >>> Angiotensin II; Saline; SC; Mice; mice (transgenic); 2004; 2 months; 7 days; Controls received mp w/ vehicle; replacement therapy (nephrectomy); long-term study; pumps replaced at 1 month; peptides; animal info (C57Bl6, female, 1 week old).

P7540 Babu,M., Purhonen,A.K., Bansiewicz,T., Maekelae,K., Walkowiak,J., Miettinen,P., Herzig,K.H. Effect of total colectomy and PYY infusion on food intake and body weight in rats. REGULATORY PEPTIDES 2005; 131(-1-3-):29-33. >>> Peptide YY (3-36); PBS; IV (jugular); Rat; 2ML2; 14 days; Controls received mp w/ vehicle; replacement therapy (proctocolectomy); no stress (see pg. 31-32) "no signs of stress... were found at any time during our studies"; peptides; post op. care (buprenorphine); animal info (male, Wistar 300-395 g); "we chose continuous infusion from subcutaneous implanted mini pumps to mimic physiological conditions and minimize stress caused by interperitoneal injections of the peptide." (p. 32).

P7534 Veiga,S., Azcoitia,I., Garcia-Segura,L.M. Extragonadal synthesis of estradiol is protective against kainic acid excitotoxic damage to the hippocampus. NeuroReport 2005; 16(-14-):1599-1603. >>> Fadrozole; Saline; SC; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (gonadectomy); enzyme inhibitor (aromatase); neurodegenerative; animal info (male and female, Wistar albino, 4 weeks old); agent also known as CGS16949A.

P7526 Toyoshima,Y., Gavrilova,O., Yakar,S., Jou,W., Pack,S., Asghar,Z., Wheeler,M.B., LeRoith,D. Leptin improves insulin resistance and hyperglycemia in a mouse model of type 2 diabetes. Endocrinology 2005; 146(-9-):4024-4035. >>> Leptin; SC; Mice; 2002; 14 days; Controls received mp w/ saline; functionality of mp verified by serum leptin levels; replacement therapy (type 2 diabetes); peptides; animal info (MKR male, 6-7 wk. old, wild type 20-25 g).

P7512 Visentin,V., Bour,S., Boucher,M., Prevot,D., Valet,P., Ordener,C., Parini,A., Carpene,C. Glucose handling in streptozotocin-induced diabetic rats is improved by tyramine but not by the amine oxidase inhibitor semicarbazide. European Journal of Pharmacology 2005; 522(-1-3-):139-146. >>> Tyramine; tyramine, 14C-; SC; Rat; 3 weeks; Controls received no treatment, normoglycemic; replacement therapy (STZ-induced diabetes); pumps replaced at 2 weeks; animal info (male, wistar, 7 wk old); amine oxidase substrate.

P7501 Seeley,R.J., Burklow,M.L., Wilmer,K.A., Matthews,C.C., Reizes,O., McOsker,C.C., Trokhan,D.P., Gross,M.C., Sheldon,R.J. The effect of the melanocortin agonist, MT-II, on the defended level of body adiposity. Endocrinology 2005; 146(-9-):3732-3738. >>> MT-II; Sodium acetate; SC; Rat; 2ML4; 28 days; Controls received mp w/ vehicle; replacement therapy (diet-induced obesity); dose-response (fig. 3); animal info (male, Wistar 275 g, 675 g); dual x-ray absortiometry; melanocortin receptor agonist.

P7492 Matasconi,M., Panini,P., Angelin,B., Rudling,M. Pituitary control of cholesterol metabolism in normal and LDL receptor knock-out mice: Effects of hypophysectomy and growth hormone treatment. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 2005; 1736(-3-):221-227. >>> Growth hormone, human; SC; Mice; 2001; 7 days; Controls received sham operation; replacement therapy (hypophysectomy); peptides; animal info (C57B1/6J, LDL R knockout, male).

P7450 Leonard,S.T., izadeh-Naderi,R., Stokes,K., Ferkin,M.H. The role of prolactin and testosterone in mediating seasonal differences in the self-grooming behavior of male meadow voles, Microtus pennsylvanicus. PHYSIOLOGY&BEHAVIOR 2005; 85(-4-):461-468. >>> Bromocriptine; prolactin, ovine; Saline, sterile; SC; Prairie vole; 2004; ; Controls received mp w/ vehicle; replacement therapy (gonadectomy); animal info (male, meadow); animals also received testosterone via silastic implant at same time.

P7443 Hasegawa,S., Watanabe,A., Nguyen,K.Q., Debonnel,G., Diksic,M. Chronic administration of citalopram in olfactory bulbectomy rats restores brain 5-HT synthesis rates: an autoradiographic study. Psychopharmacology 2005; 179(-4-):781-790. >>> Citalopram, hydrobromide; Saline; SC; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume analysis; replacement therapy (olafactory bulbectomy); animal info (male, Sprague-Dawley, 150-180 g).

P7439 Jorgensen,N.R., Teilmann,S.C., Henriksen,Z., Meier,E., Hansen,S.S., Jensen,J.E.B., Sorensen,O.H., Petersen,J.S. The antiarrhythmic peptide analog rotigaptide (ZP123) stimulates gap junction intercellular communication in human osteoblasts and prevents decrease in femoral trabecular bone strength in ovariectomized rats. Endocrinology 2005; 146(-11-):4745-4754. >>> Rotigaptide; IP; Rat; 2ML4; 30 days; Controls received mp w/ vehicle; functionality of mp verified by plasma agent levels; replacement therapy (ovariectomy); comparison of SC injections vs. mp; half-life (p. 4746) 14 days in human plasma; peptides; post op. care (temgesic, metacam); animal info (female, Wistar, 8 months old, 264 g); agent also known as ZP123.

P7432 Ellis,S., Mouihate,A., Pittman,Q.J. Early life immune challenge alters innate immune responses to lipopolysaccharide: implications for host defense as adults. FASEB Journal 2005; 19(-8-):U826-U842. >>> Corticosterone; PEG; IP; Rat; 2001; 1 week; Replacement therapy (adrenalectomy); no stress (see pg. 4); immunology; post op. care (derapen); "the survival rate of this procedure (ADX + mp) was 100% with animals having normal body temperature within 24 hours after the surgery.".

P7329 Kim,S.J., Winter,K., Nian,C., Tsuneoka,M., Koda,Y., McIntosh,C.H.S. Glucose-dependent insulinotropic polypeptide (GIP) stimulation of pancreatic beta-cell survival is dependent upon phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) signaling, inactivation of the forkhead transcription factor Foxo1, and down-regulation of bax expression. Journal of Biological Chemistry 2005; 280(-23-):22297-22307. >>> Glucose-dependent insulinotropic polypeptide (1-42), synthetic porcine; IP; Rat; 2 weeks; Replacement therapy (diabetic); immunology; peptides.

P7328 Kharitonenkov,A., Shiyanova,T.L., Koester,A., Ford,A.M., Micanovic,R., Galbreath,E.J., Sandusky,G.E., Hammond,L.J., Moyers,J.S., Owens,R.A., Gromada,J., Brozinick,J.T., Hawkins,E.D., Wroblewski,V.J., Li,D.S., Mehrbod,F., Jaskunas,S.R., Shanafelt,A.B. FGF-21 as a novel metabolic regulator. Journal of Clinical Investigation 2005; 115(-6-):1627-1635. >>> Fibroblast growth Factor-21; Saline; SC; Mice; 8 weeks; Controls received mp w/ vehicle; replacement therapy (diabetic, db/db); comparison of SC injections vs. mp (minipump used to study mitogenicity during longer exposure); long-term study.

P7319 Coppola,A., Meli,R., Diano,S. Inverse shift in circulating corticosterone and leptin levels elevates hypothalamic deiodinase type 2 in fasted rats. Endocrinology 2005; 146(-6-):2827-2833. >>> Leptin, recomb. rat; PBS; SC; Rat; 1003D; 3 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy); peptides.

P7300 Venken,K., Boonen,S., Van Herck,E., Vandenput,L., Kumar,N., Sitruk-Ware,R., Sundaram,K., Bouillon,R., Vanderschueren,D. Bone and muscle protective potential of the prostate-sparing synthetic androgen 7alpha-methyl-19-nortestosterone: Evidence from the aged orchidectomized male rat model. Bone 2005; 36(-4-):663-670. >>> Nortestosterone, 7a-methyl-19-; Propandiol; SC; Rat; 2004; 16 weeks; Controls received mp w/ vehicle; replacement therapy (orchidectomy); dose-response (fig. 1); long-term study; pumps replaced every 4 weeks; animal info (male, 13 month old, Wistar, 600-650 g).

P7274 Kulkarni,N.H., Halladay,D.L., Miles,R.R., Gilbert,L.M., Frolik,C.A., Galvin,R.J.S., Martin,T.J., Gillespie,M.T., Onyia,J.E. Effects of parathyroid hormone on Wnt signaling pathway in bone. Journal of Cellular Biochemistry 2005; 95(-6-):1178-1190. >>> Parathyroid hormone, human 1-38; PBS; serum, rat; SC; Rat; 2001D; 1, 3, 6, 24 hours; Controls received mp w/ vehicle; replacement therapy (parathyroidectomy).

P7253 Khasar,S.G., Dina,O.A., Green,P.G., Levine,J.D. Estrogen regulates adrenal medullary function producing sexual dimorphism in nociceptive threshold and beta2-adrenergic receptor-mediated hyperalgesia in the rat. European Journal of Neuroscience 2005; 21(-12-):3379-3386. >>> Epinephrine; Saline; ascorbic acid; SC; Rat; 1007D; 2002; 7, 14 days; Functionality of mp verified by plasma epinephrine levels; replacement therapy (adrenal medullectomy, adrenal gland denervation); dose-response (Fig 3).

P7225 Cassis,L.A., Helton,M.J., Howatt,D.A., King,V.L., Daugherty,A. Aldosterone does not mediate angiotensin II-induced atherosclerosis and abdominal aortic aneurysms. British Journal of Pharmacology 2005; 144(-3-):443-448. >>> Aldosterone; angiotensin II; Propylene glycol; saline; ethanol; water; SC; Mice; 2004; 28 days; Controls received mp w/ vehicle; replacement therapy (hyperlipidemic); no stress (see pg. 444); cardiovascular; 9% ethanol.

P7224 Chhokar,V.S., Sun,Y., Bhattacharya,S.K., Ahokas,R.A., Myers,L.K., Xing,Z.Q., Smith,R.A., Gerling,I.C., Weber,K.T. Hyperparathyroidism and the calcium paradox of aldosteronism. Circulation 2005; 111(-7-):871-878. >>> Aldosterone; SC; Rat; 1,2,4,6 weeks; Controls received no treatment; replacement therapy (uninephrectomy); no stress (see pg. 873); cardiovascular.

P7216 Diel,P., Laudenbach-Leschowsky,U., Friedel,A., Voss,A., Roussel,J. Pulsed estradiol exposure has a limited ability to induce uterine proliferation in ovariectomised female Wistar rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2005; 230(-1-2-):7-15. >>> Estradiol, 17B-; Saline; ethanol; SC; Rat; 10, 20 days; Replacement therapy (ovariectomy); dose-response (fig. 2); comparison of pellets vs. sc injections vs. IV injections vs. mp; "SC administration of E2 by micropump was chosen... as it provided the most constant E2 serum levels" (p. 11); animal info (F, Wistar, 130 g, OVX).

P7188 Nawate,S., Fukao,M., Sakuma,I., Soma,T., Nagai,K., Takikawa,O., Miwa,S., Kitabatake,A. Reciprocal changes in endothelium-derived hyperpolarizing factor-and nitric oxide-system in the mesenteric artery of adult female rats following ovariectomy. British Journal of Pharmacology 2005; 144(-2-):178-189. >>> Estradiol, 17B-; SC; Rat; 2ML4; 4 weeks; Controls received sham surgery; functionality of mp verified by agent plasma levels; replacement therapy (ovariectomy); cardiovascular; animal info (adult, female, OVX, Wistar, 40 weeks old).

P7177 Crowley,S.D., Gurley,S.B., Oliverio,M.I., Pazmino,A.K., Griffiths,R., Flannery,P.J., Spurney,R.F., Kim,H.S., Smithies,O., Le,T.H., Coffman,T.M. Distinct roles for the kidney and systemic tissues in blood pressure regulation by the renin-angiotensin system. Journal of Clinical Investigation 2005; 115(-4-):1092-1099. >>> Aldosterone; dexamethasone; PEG 400; saline, normal; SC; Mice; 2004; ; Replacement therapy (adrenalectomy, nephrectomy).

P7171 Bennett,E., McGuinness,L., Gevers,E.F., Thomas,G.B., Robinson,I.C.A.F., Davey,H.W., Luckman,S.M. Hypothalamic STAT proteins: Regulation, of somatostatin neurones by growth hormone via STAT5b. Journal of Neuroendocrinology 2005; 17(-3-):186-194. >>> Growth hormone, recomb.bovine; Saline; SC; Rat; 8 days; Controls received mp w/ vehicle, replacement therapy (dwarf), comparison of IP inections vs. mp.

P7160 Cettour-Rose,P., Theander-Carrillo,C., Asensio,C., Klein,M., Visser,T.J., Burger,A.G., Meier,C.A., Rohner-Jeanrenaud,F. Hypothyroidism in rats decreases peripheral glucose utilisation, a defect partially corrected by central leptin infusion. Diabetologia 2005; 48(-4-):624-633. >>> Thyroxine; leptin, human analog; triiodothyronine, reverse; Saline, isotonic; SC; Rat; 2001; 3, 6 days; Controls received mp w/ vehicle, functionality of mp verified by plasma levels, replacement therapy (hypothyroidism), enzyme inhibitor (deiodinase), peptides, multiple pumps per animal (2), agents are also known as T3 and T4.

P7142 Ruan,W.F., Monaco,M.E., Kleinberg,D.L. Progesterone stimulates mammary gland ductal morphogenesis by synergizing with and enhancing insulin-like growth factor-I action. Endocrinology 2005; 146(-3-):1170-1178. >>> Insulin-like growth factor I, des(1-3); SC; Mice; 1007D; 1002; 5, 28 days; Controls received placebo; replacement therapy (IGF-I [-/-], oophorectomy); pumps replaced at day 14; cancer (breast); endocrinology.

P7132 Brooling,J.T., Campbell,J.S., Mitchell,C., Yeoh,G.C., Fausto,N. Differential regulation of rodent hepatocyte and oval cell proliferation by interferon gamma. Hepatology 2005; 41(-4-):906-915. >>> Interferon-gamma, recomb. mouse; SC; Mice; 4-6 days; Controls received mp w/ saline; replacement therapy (hepatectomy).

P7072 Kademian,S.M.E., Bignante,A.E., Lardone,P., McEwen,B.S., Volosin,M. Biphasic effects of adrenal steroids on learned helplessness behavior induced by inescapable shock. Neuropsychopharmacology 2005; 30(-1-):58-66. >>> Aldosterone, D-; Propylene glycol; SC; Rat; 2001; 3 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy).

P6737 Sato,S., Fukushima,K., Naito,H., Funayama,Y., Suzuki,T., Sasano,H., Krozowski,Z., Shibata,C., Sasaki,I. Induction of 11 beta-hydroxysteroid dehydrogenase type 2 and hyperaldosteronism are essential for enhanced sodium absorption after total colectomy in rats. Surgery 2005; 137(-1-):75-84. >>> Aldosterone; PEG 400; SC; Rat; 2ML4; 1,2,4 weeks; Aldo plasma levels taken; replacement therapy (colectomy); no stress (see pg. 76).

P7129 Liu,Y., Imai,H., Sadamatsu,M., Tsunashima,K., Kato,N. Cytokines participate in neuronal death induced by trimethyltin in the rat hippocampus via type II glucocorticoid receptors. NEUROSCIENCE RESEARCH 2004; 50(--):209-217. >>> Aldosterone; dexamethasone; SC; Rat; 11,21 days; Controls received sham operation; replacement therapy (adrenalectomy).

P7097 Robichaud,M., Debonnel,G. Modulation of the firing activity of female dorsal raphe nucleus serotonergic neurons by neuroactive steroids. Journal of Endocrinology 2004; 182(-1-):11-21. >>> NE-100; progesterone; pregnenolone; dehydroepiandrosterone; pregnane -3,20-dione, 5B; pregnane -3-ol,20-one, 5a; pregnane -3,20-dione, 5a; pregnane -5B-ol,20-one, 3a; pregnane -3B-ol,20-one, 5B; pregnane -3B-ol,20-one, 5a; Water, distilled; ethanol; SC; CSF/CNS; Rat; 7,14,21 days; Controls received mp w/ vehicle, or saline; replacement therapy (ovariectomy); NE-100 is N,N-dipropyl-2-(4-methoxy-3-(2-phenylethoxy) phenyl)-ethylamine; allopregnanolone; DHP and THP metabolite stereoisomers.

P7093 Akirav,E.M., Chan,O., Inouye,K., Riddell,M.C., Matthews,S.G., Vranic,M. Partial leptin restoration increases hypothalamic-pituitary-adrenal activity while diminishing weight loss and hyperphagia in streptozotocin diabetic rats. METABOLISM-CLINICAL AND EXPERIMENTAL 2004; 53(-12-):1558-1564. >>> Leptin, recomb. mouse; PBS; SC; Rat; 4 days; Controls received mp w/ vehicle; replacement therapy (STZ-diabetes); peptides; STZ induced diabetes.

P7091 yala-Grosso,C., Tam,J., Xanthoudakis,S., Bureau,Y., Roy,S., Nicholson,D.W., Robertson,G.S. Effects of fimbria-fornix transection on calpain and choline acetyl transferase activities in the septohippocampal pathway. Neuroscience 2004; 126(-4-):927-940. >>> Leupeptin; MDL 28, 170; Saline, sterile; DMSO; cyclodextrin; CSF/CNS; Rat; 5 days; Controls received mp w/ vehicle; replacement therapy (axotomy); enzyme inhibitor (calpain); 2% DMSO; mp primed overnight at 37 degree Celsius.

P7088 Aubel,B., Kayser,V., Mauborgne,A., Farre,A., Hamon,M., Bourgoin,S. Antihyperalgesic effects of cizolirtine in diabetic rats: Behavioral and biochemical studies. Pain 2004; 110(-1-2-):22-32. >>> Cizolirtine; paroxetine hydrochloride; Saline; DMSO; SC; Rat; 2ML2; 2 weeks; Controls received mp w/ vehicle; replacement therapy (STZ-induced diabetes); dose-response (fig. 4); 20% DMSO.

P7084 Wu,J., Wang,X.X., Chiba,H., Higuchi,M., Nakatani,T., Ezaki,O., Cui,H.B., Yamada,K., Ishimi,Y. Combined intervention of soy isoflavone and moderate exercise prevents body fat elevation and bone loss in ovariectomized mice. METABOLISM-CLINICAL AND EXPERIMENTAL 2004; 53(-7-):942-948. >>> Estradiol, 17B-; SC; Mice; 6 weeks; Controls received no treatment; replacement therapy (ovariectomy); animal info (adult, female, ddYstrain, 27 g)).

P7076 Kim,S.W., Kim,N.N., Jeong,S.J., Munarriz,R., Goldstein,I., Traish,A.M. Modulation of rat vaginal blood flow and estrogen receptor by estradiol. Journal of Urology 2004; 172(-4-):1538-1543. >>> Estradiol, 17B-; PEG 300; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, 12-16 weeks old, 250-300g, Sprague-Dawley).

P7046 Bilodeau,M., Tousignant,J., Ethier,C., Rocheleau,B., Raymond,V.A., Lapointe,R. Anti-apoptotic effect of insulin on normal hepatocytes in vitro and in vivo. Apoptosis 2004; 9(-5-):609-617. >>> Insulin; HEPES buffer; IV (portal); Rat; 2001; 2,7 days; Controls received mp w/ vehicle; functionality of mp verified by degree of phosphorylation of insulin receptor; replacement therapy (liver atrophy); dose-response.

P7043 Ameen,C., Linden,D., Larsson,B.M., Mode,A., Holmaeng,A., Oscarsson,J. Effects of gender and GH secretory pattern on sterol regulatory element-binding protein-1c and its target genes in rat liver. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2004; 287(-6-):E1039-E1048. >>> Growth hormone, recomb. bovine; Phosphate buffer; SC; Rat; 2001; 7 days; Controls received no treatment; replacement therapy (hypophysectomy); comparison of SC injections vs. mp; peptides; "Continuous administration of GH, but not two daily injections of GH, resulted in increased triglyceride biosynthesis and VLDL assembly in isolated rat hepatocytes." (pg. E1039).

P7018 Hillisch,A., Peters,O., Kosemund,D., Mueller,G., Walter,A., Schneider,B., Reddersen,G., Elger,W., Fritzmeier,K.H. Dissecting physiological roles of estrogen receptor alpha and beta with potent selective ligands from structure-based design. MOLECULAR ENDOCRINOLOGY 2004; 18(-7-):1599-1609. >>> Estradiol, 17B-; estrogen receptor alpha ligand; estrogen receptor beta ligand; Propylene glycol; SC; Rat; 2001; 2004; 8,29 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; replacement therapy (ovariectomy); dose-response (fig 6); ERalpha ligand is an E2-1b alpha-lactone derivative (16a-LE2), ER beta ligand an E2-8 beta-vinylestra derivative (8B-VE2); E2 is 17B-estradiol; pump incorrectly labeled as a 2001D; animal info (adult, OVX, female, Wistar, 220-250 g).

P6982 Simon,F.R., Fortune,J., Iwahashi,M., Qadri,I., Sutherland,E. Multihormonal regulation of hepatic sinusoidal Ntcp gene expression. American Journal of Physiology-Gastrointestinal and Liver Physiology 2004; 287(-4-):G782-G794. >>> Growth hormone, recomb. bovine; Sodium phosphate; glycerol; sodium nitrate; SC; Rat; 2001; 7 days; Controls received sham treatment; replacement therapy (adrenalectomy, ovariectomy, hypophysectomy, thyroidectomy, castration); comparison of SC injections vs. mp; peptides.

P6978 Chu,Y.W., Memon,S.A., Sharrow,S.O., Hakim,F.T., Eckhaus,M., Lucas,P.J., Gress,R.E. Exogenous IL-7 increases recent thymic emigrants in peripheral lymphoid tissue without enhanced thymic function. Blood 2004; 104(-4-):1110-1119. >>> Interleukin-7, recomb. mouse; PBS; sucrose; serum, normal mouse; SC; Mice; 1007D; 1002; 7,14 days; Controls received mp w/ vehicle; replacement therapy (thymectomy); immunology.

P6890 Liu,I.Y.C., Lyons,W.E., Mamounas,L.A., Thompson,R.F. Brain-derived neurotrophic factor plays a critical role in contextual fear conditioning. Journal of Neuroscience 2004; 24(-36-):7958-7963. >>> Brain-derived neurotrophic factor, recomb. human; CSF, artificial; CSF/CNS (hippocampus); Mice; 1007D; 2 weeks; Controls received mp w/ vehicle; replacement therapy (BDNF knockout mice); dose-response (fig. 7); pumps replaced at the end of the 1st week; no stress (see pg. 7960); brain tissue distribution; peptides; multiple pumps per animal (2); "all mice appeared well groomed and healthy and exhibited no abnormal behaviors in their home cage." (p. 7960).

P6852 Kotokorpi,P., Gardmo,C., Nystroem,C.S., Mode,A. Activation of the glucocorticoid receptor or liver X receptors interferes with growth hormone-induced akr1b7 gene expression in rat hepatocytes. Endocrinology 2004; 145(-12-):5704-5713. >>> Growth hormone, bovine; Rat; 2001; 6 days; Replacement therapy (hypophysectomy); peptides.

P6847 Gomez-Sanchez,E.P., Ahmad,N., Romero,D.G., Gomez-Sanchez,C.E. Origin of aldosterone in the rat heart. Endocrinology 2004; 145(-11-):4796-4802. >>> Aldosterone; Rat; 8 days; Replacement therapy (adrenalectomy); cardiovascular.

P6838 Thordarson,G., Semaan,S., Low,C., Ochoa,D., Leong,H., Rajkumar,L., Guzman,R.C., Nandi,S., Talamantes,F. Mammary tumorigenesis in growth hormone deficient spontaneous dwarf rats; effects of hormonal treatments. Breast Cancer Research and Treatment 2004; 87(-3-):277-290. >>> Insulin-like growth factor I, recomb. human; SC; Rat; 21 weeks; 10 days; Controls received no treatment; replacement therapy (GH deficient, dwarf); long-term study; cancer (mammary); peptides.

P6817 Carswell,H.V.O., Macrae,I.M., Gallagher,L., Harrop,E., Horsburgh,K.J. Neuroprotection by a selective estrogen receptor beta agonist in a mouse model of global ischemia. American Journal of Physiology-Heart and Circulatory Physiology 2004; 287(-4-):H1501-H1504. >>> Diarylpropiolnitrile; propyl pyrazole triol; DMSO; saline; SC; Mice; 1002; 10 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy, ischemia); 50% DMSO used; estrogen receptor agonists; ischemia (cerebral).

P6810 Delporte,M.L., El Mkadem,S.A., Quisquater,M., Brichard,S.M. Leptin treatment markedly increased plasma adiponectin but barely decreased plasma resistin of ob/ob mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2004; 287(-3-):E446-E453. >>> Leptin, recomb. mouse; Sodium citrate; SC; Mice; 2001; 3, 6 days; Controls received mp w/ vehicle; replacement therapy (obese (ob/ob) mice); peptides; obesity; diabetes.

P6798 Brussee,V., Cunningham,F.A., Zochodne,D.W. Direct insulin signaling of neurons reverses diabetic neuropathy. Diabetes 2004; 53(-7-):1824-1830. >>> Insulin, human; FITC-Insulin; Insulin-like growth factor I; antibody, polyclonal anti-rat insulin; dye, india ink; antibody, polyclonal anti-albumin; Saline; SC; CSF/CNS (intrathecal); Rat; 2, 4 weeks; Controls received mp w/ vehicle or anti-albumin Ab; functionality of mp verified by dye; replacement therapy (streptozotocin induced diabetes); india ink used to confirm catheter placement and intrathecal delivery.

P6797 Nakamura,R., Kato,J., Kitamura,K., Onitsuka,H., Imamura,T., Cao,Y.N., Marutsuka,K., Asada,Y., Kangawa,K., Eto,T. Adrenomedullin administration immediately after myocardial infarction ameliorates progression of heart failure in rats. Circulation 2004; 110(-4-):426-431. >>> Adrenomedullin, recomb. human; Saline; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; replacement therapy (myocardial infarction); dose-response (table 1); no stress (see pg. 428); cardiovascular; peptides.

P6760 Neves,K.R., Graciolli,F.G., dos Reis,L.M., Pasqualucci,C.A., Moyses,R.M.A., Jorgetti,V. Adverse effects of hyperphosphatemia on myocardial hypertrophy, renal function, and bone in rats with renal failure. Kidney International 2004; 66(-6-):2237-2244. >>> Parathyroid hormone, rat 1-34; Cysteine; Rat; 2ML4; 8 weeks; Controls received mp w/ vehicle; replacement therapy (parathyroidectomy, 5/6 nephrectomy); long-term study; pumps replaced at day 28.

P6684 Sakuma,T., Kitajima,K., Nishiyama,M., Endo,Y., Miyauchi,K., Jarukamjorn,K., Nemoto,N. Collaborated regulation of female-specific murine Cyp3a41 gene expression by growth and glucocorticoid hormones. Biochemical and Biophysical Research Communications 2004; 314(-2-):495-500. >>> Growth hormone, recomb. human; SC; Mice; 1007D; 7 days; Functionality of mp verified by weight gain; replacement therapy (adrenalectomy); peptides.

P6639 Bains,R.K., Wells,S.E., Flavell,D.M., Fairhall,K.M., Strom,M., Le Tissier,P., Robinson,I.C.A.F. Visceral obesity without insulin resistance in late-onset obesity rats. Endocrinology 2004; 145(-6-):2666-2679. >>> Growth hormone, human; SC; Rat; 4 weeks; Controls received no treatment; replacement therapy (GH deficiency); pumps replaced on day 13; peptides.

P6617 Katsumata,S., Masuyama,R., Koshihara,M., Matsuzaki,H., Uehara,M., Suzuki,K. High phosphorus diet changes phosphorus metabolism regardless of PTH action in rats. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2004; 68(-1-):243-246. >>> Parathyroid hormone (1-34); Saline; SC; Rat; 2002; ; Controls received mp w/ vehicle; PTH plasma levels taken; replacement therapy (parathyroidectomy); peptides.

P6605 Gertner,R.A., Klein,J.D., Bailey,J.L., Kim,D.U., Luo,X.H., Bagnasco,S.M., Sands,J.M. Aldosterone decreases UT-A1 urea transporter expression via the mineralocorticoid receptor. Journal of the American Society of Nephrology 2004; 15(-3-):558-565. >>> Aldosterone; spironolactone; dexamethasone; Saline; DMSO; SC; Rat; 14 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy);<10% DMSO used.

P6581 Pazol,K., Wilson,M.E., Wallen,K. Medroxyprogesterone acetate antagonizes the effects of estrogen treatment on social and sexual behavior in female macaques. Journal of Clinical Endocrinology and Metabolism 2004; 89(-6-):2998-3006. >>> Progesterone acetate, medroxy-; progesterone; DMSO; SC; Monkey; 2ML1; 1 week; Controls received placebo; functionality of mp verified by serum hormone levels; replacement therapy (ovariectomy); pumps replaced animals received mp w/ agent 1 for 1 week, mp removed, 3 week washout period and then new mp w/ agent 2 for 1 week; peptides.

P6566 Li,Y.H., Kishimoto,I., Saito,Y., Harada,M., Kuwahara,K., Izumi,T., Hamanaka,I., Takahashi,N., Kawakami,R., Tanimoto,K., Nakagawa,Y., Nakanishi,M., Adachi,Y., Garbers,D.L., Fukamizu,A., Nakao,K. Androgen contributes to gender-related cardiac hypertrophy and fibrosis in mice lacking the gene encoding guanylyl cyclase-A. Endocrinology 2004; 145(-2-):951-958. >>> Flutamide; PEG 300; SC; Mice; 2002; 6 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy, castration); pumps replaced every 2 weeks; cardiovascular.

P6561 Zhang,M.Z., Lopez,P.S., McKanna,J.A., Harris,R.C. Regulation of cyclooxygenase expression by vasopressin in rat renal medulla. Endocrinology 2004; 145(-3-):1402-1409. >>> Vasopressin, arginine-8-; Vasopressin, 1-desamino-8-D arginine; PV-OT; D3PVP; Water, distilled; SC; Rat; 2002; 6 days; Controls received mp w/ vehicle; replacement therapy (hypophysectomy); dose-response (table 1); comparison of IM or IP injections vs. mp; PV-OT is a V1a receptor agonist, D3PVP is a Vlb receptor agonist; dDVAP is a V2 receptor agonist.

P6545 Cheng,X., Kinosaki,M., Takami,M., Choi,Y., Zhang,H.T., Murali,R. Disabling of receptor activator of nuclear factor-kappa B (RANK) receptor complex by novel osteoprotegerin-like peptidomimetics restores bone loss in vivo. Journal of Biological Chemistry 2004; 279(-9-):8269-8277. >>> OP3-4; SC; Mice; 2004; 28 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); OP3-4 is an osteoprotegerin mimetic; "Because of the limited solubility of OP3-4 and to avoid any effect resulting from peptide aggregation, which might occur at high doses, we chose to deliver OP3-4 using an ALZET pump. The advantage of using the pump is that it can offer a consistant administration at low dosing." (p. 8274).

P6544 Buffin-Meyer,B., Younes-Ibrahim,M., El Mernissi,G., Cheval,L., Marsy,S., Grima,M., Girolami,J.P., Doucet,A. Differential regulation of collecting duct Na+,K+-ATPase and K+ excretion by furosemide and piretanide: Role of bradykinin. Journal of the American Society of Nephrology 2004; 15(-4-):876-884. >>> Furosemide; dexmethasone; diretanide; aldosterone; Hoe 140; Saline; SC; IP; Rat; 6 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy); solution was a pH 9.0; a third group was adrenalectomized and given dexamethasone and aldosterone by a SC ALZET pump; in a fifth set of experiments Hoe140 was infused SC.

P6517 Kim,N.N., Min,K., Pessina,M.A., Munarriz,R., Goldstein,I., Traish,A.M. Effects of ovariectomy and steroid hormones on vaginal smooth muscle contractility. International Journal of Impotence Research 2004; 16(-1-):43-50. >>> Testosterone; estradiol; dihydrotestosterone; dehydroepiandrosterone; PEG 300; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, New Zealand, white, 4.5-5.0 kg, OVX (for some)).

P6433 Zhang,R.X., La,L.X., Qiao,J.T., Malsnee,K., Ruda,M.A. Endogenous and exogenous glucocorticoid suppresses up-regulation of preprodynorphin mRNA and hyperalgesia in rats with peripheral inflammation. Neuroscience Letters 2004; 359(-1-2-):85-88. >>> Dexamethasone; Propylene glycol; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; replacement therapy (adrenalectomy); half-life (p. 86) 36-72 hours.

P6410 Onyia,J.E., Galvin,R.J.S., Ma,Y.F.L., Halladay,D.L., Miles,R.R., Yang,X.H., Fuson,T., Cain,R.L., Zeng,Q.Q., Chandrasekhar,S., Emkey,R., Xu,Y.P., Thirunavukkarasu,K., Bryant,H.U., Martin,T.J. Novel and selective small molecule stimulators of osteoprotegerin expression inhibit bone resorption. Journal of Pharmacology and Experimental Therapeutics 2004; 309(-1-):369-379. >>> Parathyroid hormone, human; Saline; serum; SC; Rat; 56 hours; Replacement therapy (parathyroidectomy); pump model not listed.

P6393 Bhargava,A., Wang,J., Pearce,D. Regulation of epithelial ion transport by aldosterone through changes in gene expression. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2004; 217(-1-2-):189-196. >>> Aldosterone; SC; Rat; 24 hours; Controls received mp w/ vehicle; replacement therapy (adrenalectomy); half-life (p. 192), 20 minutes; pump model not listed.

P6387 Matasconi,M., Angelin,B., Rudling,M. Pituitary control of lipoprotein and bile acid metabolism in male rats: growth hormone effects are not mediated by prolactin. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2004; 287(-1-):E114-E119. >>> Growth hormone, human; growth hormone, bovine; SC; Rat; 2001; 7 days; Replacement therapy (hyphophysectomy); dose-response (p. E115).

P6356 Cheng,G.J., Zhang,W.H., Warner,M., Gustafsson,J.A. Estrogen receptors ER alpha and ER beta in proliferation in the rodent mammary gland. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-11-):3739-3746. >>> Estradiol-17 beta; tamoxifen; Intralipid; IP; Mice; 2004; 48 hours; Controls received mp w/ vehicle; replacement therapy (ovariectomy).

P6288 Quesada,A., Micevych,P.E. Estrogen interacts with the IGF-1 system to protect nigrostriatal dopamine and maintain motoric behavior after 6-hydroxdopamine lesions. Journal of Neuroscience Research 2004; 75(-1-):107-116. >>> Insulin-like factor 1; JB-1; CSF, artificial; SC; CSF/CNS (medial forebrain); Rat; 1007D; 7 days; Comparison of sc infusion vs icv infusion; ALZET brain infusion kit 2; parkinson's disease; JB-1 is an IGF-1 receptor antagonist; behavorial study; neurodegenerative (Parkinson's disease); replacement therapy (ovariectomy).

P6273 Traish,A.M., Huang,Y.H., Min,K.S., Kim,N.N., Munarriz,R., Goldstein,I. Binding characteristics of [3H]Delta5-androstene-3beta 17beta-diol to a nuclear protein in the rabbit vagina. Steroids 2004; 69(-1-):71-78. >>> Estradiol; testosterone; PEG; SC; Rabbit; 2002; 2 weeks; Replacement therapy (ovariectomy); animal info (female, New Zealand, white, 4.5-5.0 kg, OVX).

P6260 Gibbs,R.B., Gabor,R., Cox,T., Johnson,D.A. Effects of raloxifene and estradiol on hippocampal acetylcholine release and spatial learning in the rat. Psychoneuroendocrinology 2004; 29(-6-):741-748. >>> Raloxifene; estradiol, 17 beta; DMSO; cyclodextrin, hydroxypropyl-beta (molecusol); SC; Rat; 2004; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); pumps replaced every 28 days; 20% cyclodextrin&1% DMSO used; incorrectly states pumping rate as 2.5 ul/hr; behavioral study; research diets.

R0201 Sun,Y., Weber,K.T. RAS and connective tissue in the heart. INTERNATIONAL JOURNAL OF BIOCHEMISTRY&CELL BIOLOGY 2003; 35(-6-):919-931. >>> Angiotensin II; aldosterone; Rat; 3 weeks; Replacement therapy (uninephrectomy); cardiovascular.

R0200 Samuelson,L.C., Hinkle,K.L. Insights into the regulation of gastric acid secretion through analysis of genetically engineered mice. Annual Review of Physiology 2003; 65(--):383-400. >>> Gastrin; Mice; 6 days; Replacement therapy (gastrin deficient mice).

P7431 Ray,E.C., Avissar,N.E., Vukcevic,D., Toia,L., Ryan,C.K., Berlanga-Acosta,J., Sax,H.C. Growth hormone and epidermal growth factor together enhance amino acid transport systems B0,+ and A in remnant small intestine after massive enterectomy. J.Surg.Res. 2003/8; 113(-2-):257-263. >>> Epidermal growth factor; Albumin; SC; Rabbit; 2 weeks; Controls received mp w/ vehicle; replacement therapy (enterectomy); no stress (see pg. 258-259).

P6827 Miyazaki,M., Jacobson,M.J., Man,W.C., Cohen,P., Asilmaz,E., Friedman,J.M., Ntambi,J.M. Identification and characterization of murine SCD4, a novel heart-specific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. Journal of Biological Chemistry 2003; 278(-36-):33904-33911. >>> Leptin, recomb. mouse; PBS; SC; Mice; 1002; 12 days; Controls received mp w/ vehicle; replacement therapy (ob/ob mice); cardiovascular; peptides.

P6588 Min,K., Munarriz,R., Kim,N.N., Choi,S., O'Connell,L., Goldstein,I., Traish,A.M. Effects of ovariectomy and estrogen replacement on basal and pelvic nerve stimulated vaginal lubrication in an animal model. JOURNAL OF SEX&MARITAL THERAPY 2003; 29(-1-):77-84. >>> Estradiol; PEG 300; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; replacement therapy (ovariectomy); post op. care (butorphanol tartrate, cephazolin).

P6506 Nagano,M., Sakai,A., Takahashi,N., Umino,M., Yoshioka,K., Suzuki,H. Decreased expression of glial cell line-derived neurotrophic factor signaling in rat models of neuropathic pain. British Journal of Pharmacology 2003; 140(-7-):1252-1260. >>> Glial-derived neurotrophic factor, recomb. human; Saline; CSF/CNS (intrathecal); Rat; 9 days; Controls received mp w/ vehicle; replacement therapy (spinal nerve ligation axotomy); post op. care (IP kanamycin sulfate).

P6480 Traish,A.M., Kim,N.N., Huang,Y.H., Min,K., Munarriz,R., Goldstein,I. Sex steroid hormones differentially regulate nitric oxide synthase and arginase activities in the proximal and distal rabbit vagina. International Journal of Impotence Research 2003; 15(-6-):397-404. >>> Testosterone; dehydroepiandrosterone; dihydrotestosterone, 5-alpha-; androstenediol, delta 5-3B, 17B; estradiol; progesterone; PEG; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); multiple pumps per animal (2).

P6334 Ameen,C., Oscarsson,J. Sex difference in hepatic microsomal triglyceride transfer protein expression is determined by the growth hormone secretory pattern in the rat. Endocrinology 2003; 144(-9-):3914-3921. >>> Growth hormone, recomb. bovine; Phosphate buffer; glycerol; sodium azide; SC; Rat; 2001; 7 days; Controls were age matched normal and Hx rats; replacement therapy (hypophysectomy); comparison of sc injections vs. mp.

P6247 Suda,T., Ueno,Y., Fujii,K., Shinki,T. Vitamin D and bone. Journal of Cellular Biochemistry 2003; 88(-2-):259-266. >>> Parathyroid hormone; Rat; 14 days; Functionality of mp verified by serum PTH levels; replacement therapy (thyroparathyroidectomy); peptides.

P6246 Staal,A., Frith,J.C., French,M.H., Swartz,J., Gungor,T., Harrity,T.W., Tamasi,J., Rogers,M.J., Feyen,J.H. The ability of statins to inhibit bone resorption is directly related to their inhibitory effect on HMG-CoA reductase activity. Journal of Bone and Mineral Research 2003; 18(-1-):88-96. >>> Parathyroid hormone, human 1-34; SC; Rat; 3 days; Functionality of mp verified by plasma Ca concentration; replacement therapy (Thyro-parathyroidectomy); peptides.

P6237 Watanobe,H., Habu,S. Adrenal glucocorticoids do not mediate impaired reproductive function induced by lipopolysaccharide in rats. Neuroendocrinology 2003; 78(-1-):23-28. >>> Corticosterone; PEG 400; SC; Rat; 2ML2; 14 days; Controls received no mp and saline injection; functionality of mp verified by plasma drug concentrations; replacement therapy (orchidectomy; adrenalectomy); LPS given by injection.

P6236 Kovacs,P., Morales,J.C., Karkanias,G.B. Central insulin administration maintains reproductive behavior in diabetic female rats. Neuroendocrinology 2003; 78(-2-):90-95. >>> Insulin, human; Saline; CSF/CNS (third ventricle); Rat; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); no stress (see pg. 91).

P6227 Davis,T.L., Mussard,M.L., Jimenez-Severiano,H., Enright,W.J., Kinder,J.E. Chronic treatment with an agonist of gonadotropin-releasing hormone enhances luteal function in cattle. Biology of Reproduction 2003; 69(-2-):398-403. >>> Azagly-NaFarelin; Sodium Chloride; SC; Cattle; 2ML2; 2ML4; 9, 18 days; Controls received no treatment; replacement therapy (ovariectomy); agent is a gonadotrophin-releasing hormone agonist.

P6120 Krug,A.W., Papavassiliou,F., Hopfer,U., Ullrich,K.J., Gekle,M. Aldosterone stimulates surface expression of NHE3 in renal proximal br ush borders. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 2003; 446(-4-):492-496. >>> Aldosterone; dexamethasone; SC; Rat; 2ML1; 5 days; Functionality of mp verified by aldosterone plasma levels; replacement therapy (adrenalectomy).

P6109 Wang,X.X., Wu,J., Chiba,H., Umegaki,K., Yamada,K., Ishimi,Y. Puerariae radix prevents bone loss in ovariectomized mice. JOURNAL OF BONE AND MINERAL METABOLISM 2003; 21(-5-):268-275. >>> Estradiol, 17B; SC; Mice; 4 weeks; Replacement therapy (ovariectomy); pump model not stated.

P6034 Krug,A.W., Grossmann,C., Schuster,C., Freudinger,R., Mildenberger,S., Govindan,M.V., Gekle,M. Aldosterone stimulates epidermal growth factor receptor expression. Journal of Biological Chemistry 2003; 278(-44-):43060-43066. >>> Aldosterone; SC; Rat; 2ML1; 1 week; Controls received mp w/ vehicle; aldosterone plasma levels taken; replacement therapy (adrenalectomy).

P6016 Veiga,S., Garcia-Segura,L.M., Azcoitia,I. Neuroprotection by the steroids pregnenolone and dehydroepiandrosterone is mediated by the enzyme aromatase. Journal of Neurobiology 2003; 56(-4-):398-406. >>> Pregnenolone; dehydroepiandrosterone; Saline; fadrozole; SC; Rat; 2002; ; Controls received mp w/ vehicle; replacement therapy (gonadectomy); comparison of IP injections vs. chronic mp; dehydroepiandrosterone (DHEA) and pregnenolone are sex hormone precursors.

P5985 de la Rosa,D.A., Coric,T., Todorovic,N., Shao,D.R., Wang,T., Canessa,C.M. Distribution and regulation of expression of serum- and glucocorticoid-induced kinase-1 in the rat kidney. JOURNAL OF PHYSIOLOGY-LONDON 2003; 551(-2-):455-466. >>> Dexamethasone; SC; Rat; 2002; 1 week; Replacement therapy (adrenalectomy).

P5934 Chander,P.N., Rocha,R., Ranaudo,J., Singh,G., Zuckerman,A., Stier,CT Jr  Journal of the American Society of Nephrology 2003; 14(-8-):1990-1997. >>> Angiotensin II; aldosterone; Ethanol; SC; Rat; 2 weeks; Replacement therapy (adrenalectomy); pump model not stated.

P5931 Momoi,H., Ikomi,F., Ohhashi,T. Estrogen-induced augmentation of endothelium- dependent nitric oxide-mediated vasodilation in isolated rat cerebral small arteries. Japanese Journal of Physiology 2003; 53(-3-):193-203. >>> Estradiol, 17B; Polypropylene glycol; SC; Rat; 2002; 7 days; Replacement therapy (ovariectomy).

P5927 Shao,T.C., Li,H.L., Eid,W., Ittmann,M., Unni,E., Cunningham,G.R. In vivo preservation of steroid specificity in CWR22 xenografts having a mutated androgen receptor. PROSTATE 2003; 57(-1-):1-7. >>> Testosterone; estradiol; progesterone; flutamide; Cylodextrin, 2-beta-hydroxypropl; SC; Mice (nude); 2004; 4 weeks; Replacement therapy (castration); cancer (prostate); CWR22 xenograft used; flutamide is an anti-androgen; animal info (5-6 week old, nude ,ORX).

P5894 Jalouli,M., Carlsson,L., Ameen,C., Linden,D., Ljungberg,A., Michalik,L., Eden,S., Wahli,W., Oscarsson,J. Sex difference in hepatic peroxisome proliferator-activated receptor alpha expression: Influence of pituitary and gonadal hormones. Endocrinology 2003; 144(-1-):101-109. >>> Growth hormone, bovine; testosterone; estradiol, 17B-; Phosphate buffer; glycerol; sodium asize; propylene glycol; SC; Rat; 2001; 7 days; Replacement therapy (gonadectomy); comparison of daily injections vs. chronic mp; peptides; GH was recomb bovine&diluted in 0.05 m phosphate buffer, ph 8.6, with 1.6% glycerol&0.02% sodium azide; testosterone&estradiol were diluted in propylene glycol.

P5857 Wotus,C., Engeland,W.C. Differential regulation of adrenal corticosteroids after restriction-induced drinking in rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2003; 284(-1-):R183-R191. >>> Aldosterone; Propylene glycol; SC; Rat; ; Controls received mp w/ vehicle; replacement therapy (adrenalectomy).

P5855 Wu,J., Wang,X.X., Chiba,H., Higuchi,M., Takasaki,M., Ohta,A., Ishimi,Y. Combined intervention of exercise and genistein prevented androgen deficiency-induced bone loss in mice. Journal of Applied Physiology 2003; 94(-1-):335-342. >>> Genistein; Estradiol, 17B-; DMSO; PEG 300; SC; Mice; 2002; 4 weeks; Replacement therapy (orchidectomy); dose-response (p.336); 20% DMSO used in vehicle.

P5720 Jiang,G.R., Cobbs,S., Klein,J.D., O'Neill,W.C. Aldosterone regulates the Na-K-2Cl cotransporter in vascular smooth muscle. Hypertension 2003; 41(-5-):1131-1135. >>> Aldosterone; DMSO; saline; SC; Rat; 2001; 7 days; Controls received mp w/ vehicle; serum aldosterone levels checked; replacement therapy (adrenalectomy); cardiovascular; 10% DMSO used.

P5700 Jesmin,S., Hattori,Y., Sakuma,I., Liu,M.Y., Mowa,C.N., Kitabatake,A. Estrogen deprivation and replacement modulate cerebral capillary density with vascular expression of angiogenic molecules in middle-aged female rats. Journal of Cerebral Blood Flow and Metabolism 2003; 23(-2-):181-189. >>> Estradiol, 17B-; SC; Rat; 2ML4; ; Replacement therapy (ovariectomy).

P5670 Eckardt,H., Bundgaard,K.G., Christensen,K.S., Lind,M., Hansen,E.S., Hvid,I. Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF-inhibitor to the rabbit tibia during distraction osteogenesis. Journal of Orthopaedic Research 2003; 21(-2-):335-340. >>> Vascular endothelial growth factor; vascular endothelial growth factor R2/FC chimera; Phosphate buffer; BSA; Bone (tibia); Rabbit; 2ML2; 14 days; Controls received mp w/ vehicle; tissue perfusion (osteotomy site); replacement therapy (distraction osteogenesis); stability verified by in vitro assay for 2 weeks at 37 C. (p. 336);.

P5619 Yin,D.H., Xu,H.P., He,Y.L., Kirkovsky,L.I., Miller,D.D., Dalton,J.T. Pharmacology, pharmacokinetics, and metabolism of acetothiolutamide, a novel nonsteroidal agonist for the androgen receptor. Journal of Pharmacology and Experimental Therapeutics 2003; 304(-3-):1323-1333. >>> Testosterone propionate; acetothiolutamide; bicalutamide, R-; PEG 300; SC; Rat; 2002; 2 weeks; Controls received mp w/vehicle; functionality of mp verified by residual volume; replacement therapy (orchidectomy); comparison of bolus IV injections vs. mp (p. 1327); stability verified by preliminary experiments (14 days at 37 C. of acetothiolutamide; half life (p. 1327) acetothiolutamide (26 min); bicalutamide is a nonsteroidal androgen; acetothioluthamide is a nonsteroidal androgen receptor ligand; agents in seperate pumps for seperate groups..

P5613 Chen,H.C., Jensen,D.R., Myers,H.M., Eckel,R.H., Farese,RV Jr  Journal of Clinical Investigation 2003; 111(-11-):1715-1722. >>> Formoterol fumarate; Saline; SC; Rat (pregnant); 2ML1; 5 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); dose-response (p.165); teratology; formoterol is a highly potent, long lasting B2-adrenoceptor agonist used for asthma therapy..

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