References on the Administration of Estrogens Using ALZET^® Osmotic Pumps

P8500 Xue,B.J., Pamidimukkala,J., Lubahn,D.B., Hay,M. Estrogen receptor-alpha mediates estrogen protection from angiotensin II- induced hypertension in conscious female. American Journal of Physiology-Heart and Circulatory Physiology 2007; 292(-4-):H1770-H1776. >>> Angiotensin II; estradiol, 17 beta; ICI-182, 780; CSF/CNS; SC; Mice; 1002; ; Blood pressure measured; cardiovascular; ALZET brain infusion kit used; peptides; animal info (OVX, WT, and ER knockout); CNS group delivered E2 + ICI.

P8475 Huber,C., Collishaw,S., Mosley,J.R., Reeve,J., Noble,B.S. Selective estrogen receptor modulator inhibits osteocyte apoptosis during abrupt estrogen withdrawal: Implications for bone quality maintenance. Calcified Tissue International 2007; 81(-2-):139-144. >>> Estradiol, 17 beta; LY117018; Cyclodextrin, beta-hydroxy; SC; Rat; 7, 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, Sprague-Dawley, 250-270 g).

P8405 Zhang,X.C., Jia,Y.H., Jackson,E.K., Tofovic,S.P. 2-methoxyestradiol and 2-ethoxyestradiol retard the progression of renal disease in aged, obese, diabetic ZSF1 rats. Journal of Cardiovascular Pharmacology 2007; 49(-1-):56-63. >>> Estradiol, 2-hydroxy-; estradiol, 1-methoxy-; PEG 400; Rat; 2ML4; 9 weeks; Controls received mp w/ vehicle; long-term study; pumps replaced after 32 days; no stress (see pg. 58-9); cardiovascular; animal info (male, obese ZSF1,35 wks old, 650-375g.); nephrology.

P8164 Barchiesi,F., Jackson,E.K., Fingerle,J., Gillespie,D.G., Odermatt,B., Dubey,R.K. 2-methoxyestradiol, an estradiol metabolite, inhibits neointima formation and smooth muscle cell growth via double blockade of the cell cycle. Circulation Research 2006; 99(-3-):266-274. >>> Estradiol, 2-methoxy-; IV; Rat; 7, 8, 14, days; Controls received mp w/ vehicle; no stress (see pg. 269); cardiovascular; toxicology; animal info (male, Wistar Kyoto, 350-400 grams); balloon carotid artery injury, "treatment with 2-ME was not associated with any toxic adverse effects." (p. 269); estradiol metabolite.

P8140 Husen,B., Huhtinen,K., Saloniemi,T., Messinger,J., Thole,H.H., Poutanen,M. Human hydroxysteroid (17-beta) dehydrogenase 1 expression enhances estrogen sensitivity of MCF-7 breast cancer cell xenografts. Endocrinology 2006; 147(-11-):5333-5339. >>> B10721325; estrone; DMSO; propanediol; SC; Mice (nude); 1002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); dose-response (p.5335); pumps replaced after 14 days; enzyme inhibitor (hydroxysteroid 17 beta dehydrogenase 1); cancer (breast); animal info (female, BALB/cA Bom-v, Crl: nu/nu, 10-11 wks old); 50%DMSO; endocrinology.

P7993 Vandenberg,L.N., Wadia,P.R., Schaeberle,C.M., Rubin,B.S., Sonnenschein,C., Soto,A.M. The mammary gland response to estradiol: Monotonic at the cellular level, non-monotonic at the tissue-level of organization?. Journal of Steroid Biochemistry and Molecular Biology 2006; 101(-4-5-):263-274. >>> Estradiol, 17 beta; DMSO; SC; Mice; 1002; 10 days; Controls received mp w/ vehicle; replacement therapy (OVX); dose-response (267); animal info (female, CD-1, OVX); 50% DMSO used.

P7902 Husen,B., Huhtinen,K., Poutanen,M., Kangas,L., Messinger,J., Thole,H. Evaluation of inhibitors for 17 beta-hydroxysteroid dehydrogenase type 1 in vivo in immunodeficient mice inoculated with MCF-7 cells stably expressing the recombinant human enzyme. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2006; 248(-1-2-):109-113. >>> Estrone; B10720512; B10721325; B10720440; B10720511; B10715817; DMSO; propanediol; SC; Mice (nude); 1002; 4 weeks; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); dose-response (fig. 5); pumps replaced after 2 weeks; enzyme inhibitor (17 beta-hydroxysteroid dehydrogenase); cancer (breast carcinoma); animal info (female, Crl: NU/NU, 10-11 wk old); 50% DMSO.

P7573 Mark,P.J., Smith,J.T., Waddell,B.J. Placental and fetal growth retardation following partial progesterone withdrawal in rat pregnancy. PLACENTA 2006; 27(-2-3-):208-214. >>> Estradiol; Propylene glycol; SC; Rat (pregnant); 6 days; Controls received sham operation and 2x daily injection of vehicle; functionality of mp verified by plasma estradiol concentrations; replacement therapy (ovariectomy); animal info (female, albino Wistar, 3-5 month old).

P7678 Guan,G.Q., Kerins,C.C., Bellinger,L.L., Kramer,P.R. Estrogenic effect on swelling and monocytic receptor expression in an arthritic temporomandibular joint model. Journal of Steroid Biochemistry and Molecular Biology 2005; 97(-3-):241-250. >>> Estradiol, 17B; PEG; Rat; 2002; 14 days; Animal info (OVX, Sprague-Dawley, 200-225 g, female).

P7644 Wang,X.X., Wu,J., Chiba,H., Yamada,K., Ishimi,Y. Puerariae radix prevents bone loss in castrated male mice. METABOLISM-CLINICAL AND EXPERIMENTAL 2005; 54(-11-):1536-1541. >>> Estradiol, 17B; SC; Mice; 4 weeks; Controls received sham surgery with no mp; replacement therapy (orchidectomy); animal info (male, ddY, 8 week old).

P7634 Andersson,N., Islander,U., Egecioglu,E., Loef,E., Swanson,C., Moverare-Skrtic,S., Sjoegren,K., Lindberg,M.K., Carlsten,H., Ohlsson,C. Investigation of central versus peripheral effects of estradiol in ovariectomized mice. Journal of Endocrinology 2005; 187(-2-):303-309. >>> Estradiol, 17B; Cyclodextrin, hydroxypropyl, beta; CSF/CNS; SC; Mice; 1002; ; Controls received mp w/ vehicle; ALZET Brain Infusion Kit used; multiple pumps per animal (2) used; animal info (F, OVX, C57BL/6, 8 weeks old); SC and ICV administration performed concomitantly.

P7601 Batista,M.R., Smith,M.S., Snead,W.L., Connolly,C.C., Lacy,D.B., Moore,M.C. Chronic estradiol and progesterone treatment in conscious dogs: effects on insulin sensitivity and response to hypoglycemia. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2005; 289(-4-):R1064-R1073. >>> Estradiol; Saline; SC; Dog; 2ML4; 3 weeks; Controls received mp w/ vehicle; no stress (see pg. R1065); animal info (female, mongrel, 1 yr, hypoglycemia).

P7549 Munoz-de-Toro,M., Markey,C.M., Wadia,P.R., Luque,E.H., Rubin,B.S., Sonnenschein,C., Soto,A.M. Perinatal exposure to bisphenol-A alters peripubertal mammary gland development in mice. Endocrinology 2005; 146(-9-):4138-4147. >>> Bisphenol A; estradiol; DMSO; SC; Mice (pregnant); 10, 14 days; Controls received mp w/ vehicle; dose-response (fig. 1); cancer (mammary); teratology; animal info (female, CD-1, 9 wk old).

P7216 Diel,P., Laudenbach-Leschowsky,U., Friedel,A., Voss,A., Roussel,J. Pulsed estradiol exposure has a limited ability to induce uterine proliferation in ovariectomised female Wistar rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2005; 230(-1-2-):7-15. >>> Estradiol, 17B-; Saline; ethanol; SC; Rat; 10, 20 days; Replacement therapy (ovariectomy); dose-response (fig. 2); comparison of pellets vs. sc injections vs. IV injections vs. mp; "SC administration of E2 by micropump was chosen... as it provided the most constant E2 serum levels" (p. 11); animal info (F, Wistar, 130 g, OVX).

P7200 Robichaud,M., Debonnel,G. Oestrogen and testosterone modulate the firing activity of dorsal raphe nucleus serotonergic neurones in both male and female rats. Journal of Neuroendocrinology 2005; 17(-3-):179-185. >>> Estradiol, 17B-; testosterone; progesterone; pregnane-3, 20 dione, 5B-; pregnane-3a-ol, 20-one, 5a-; dehydroepiandrosterone; testosterone, 5a-dihydroxy; Ethanol; water, distilled; CSF/CNS; Rat; 2ML1; 3, 7 days; Controls received mp w/ vehicle; ALZET brain infusion kit used; 3% ethanol; animal info (Sprague-Dawley, 250-325 g).

P7188 Nawate,S., Fukao,M., Sakuma,I., Soma,T., Nagai,K., Takikawa,O., Miwa,S., Kitabatake,A. Reciprocal changes in endothelium-derived hyperpolarizing factor-and nitric oxide-system in the mesenteric artery of adult female rats following ovariectomy. British Journal of Pharmacology 2005; 144(-2-):178-189. >>> Estradiol, 17B-; SC; Rat; 2ML4; 4 weeks; Controls received sham surgery; functionality of mp verified by agent plasma levels; replacement therapy (ovariectomy); cardiovascular; animal info (adult, female, OVX, Wistar, 40 weeks old).

P6965 Soustiel,J.F., Palzur,E., Nevo,O., Thaler,I., Vlodavsky,E. Neuroprotective anti-apoptosis effect of estrogens in traumatic brain injury. Journal of Neurotrauma 2005; 22(-3-):345-352. >>> Estrogen, conjugated; SC; Rat; 3 days; Traumatic brain injury; neuroprotection; animal info (male, Sprague-Dawley).

P7084 Wu,J., Wang,X.X., Chiba,H., Higuchi,M., Nakatani,T., Ezaki,O., Cui,H.B., Yamada,K., Ishimi,Y. Combined intervention of soy isoflavone and moderate exercise prevents body fat elevation and bone loss in ovariectomized mice. METABOLISM-CLINICAL AND EXPERIMENTAL 2004; 53(-7-):942-948. >>> Estradiol, 17B-; SC; Mice; 6 weeks; Controls received no treatment; replacement therapy (ovariectomy); animal info (adult, female, ddYstrain, 27 g)).

P7076 Kim,S.W., Kim,N.N., Jeong,S.J., Munarriz,R., Goldstein,I., Traish,A.M. Modulation of rat vaginal blood flow and estrogen receptor by estradiol. Journal of Urology 2004; 172(-4-):1538-1543. >>> Estradiol, 17B-; PEG 300; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, 12-16 weeks old, 250-300g, Sprague-Dawley).

P7018 Hillisch,A., Peters,O., Kosemund,D., Mueller,G., Walter,A., Schneider,B., Reddersen,G., Elger,W., Fritzmeier,K.H. Dissecting physiological roles of estrogen receptor alpha and beta with potent selective ligands from structure-based design. MOLECULAR ENDOCRINOLOGY 2004; 18(-7-):1599-1609. >>> Estradiol, 17B-; estrogen receptor alpha ligand; estrogen receptor beta ligand; Propylene glycol; SC; Rat; 2001; 2004; 8,29 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; replacement therapy (ovariectomy); dose-response (fig 6); ERalpha ligand is an E2-1b alpha-lactone derivative (16a-LE2), ER beta ligand an E2-8 beta-vinylestra derivative (8B-VE2); E2 is 17B-estradiol; pump incorrectly labeled as a 2001D; animal info (adult, OVX, female, Wistar, 220-250 g).

P6517 Kim,N.N., Min,K., Pessina,M.A., Munarriz,R., Goldstein,I., Traish,A.M. Effects of ovariectomy and steroid hormones on vaginal smooth muscle contractility. International Journal of Impotence Research 2004; 16(-1-):43-50. >>> Testosterone; estradiol; dihydrotestosterone; dehydroepiandrosterone; PEG 300; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); animal info (female, New Zealand, white, 4.5-5.0 kg, OVX (for some)).

P6356 Cheng,G.J., Zhang,W.H., Warner,M., Gustafsson,J.A. Estrogen receptors ER alpha and ER beta in proliferation in the rodent mammary gland. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004; 101(-11-):3739-3746. >>> Estradiol-17 beta; tamoxifen; Intralipid; IP; Mice; 2004; 48 hours; Controls received mp w/ vehicle; replacement therapy (ovariectomy).

P6273 Traish,A.M., Huang,Y.H., Min,K.S., Kim,N.N., Munarriz,R., Goldstein,I. Binding characteristics of [3H]Delta5-androstene-3beta 17beta-diol to a nuclear protein in the rabbit vagina. Steroids 2004; 69(-1-):71-78. >>> Estradiol; testosterone; PEG; SC; Rabbit; 2002; 2 weeks; Replacement therapy (ovariectomy); animal info (female, New Zealand, white, 4.5-5.0 kg, OVX).

P6260 Gibbs,R.B., Gabor,R., Cox,T., Johnson,D.A. Effects of raloxifene and estradiol on hippocampal acetylcholine release and spatial learning in the rat. Psychoneuroendocrinology 2004; 29(-6-):741-748. >>> Raloxifene; estradiol, 17 beta; DMSO; cyclodextrin, hydroxypropyl-beta (molecusol); SC; Rat; 2004; ; Controls received mp w/ vehicle; replacement therapy (ovariectomy); pumps replaced every 28 days; 20% cyclodextrin&1% DMSO used; incorrectly states pumping rate as 2.5 ul/hr; behavioral study; research diets.

P6259 Yokota,T., Honda,K., Tsuruya,Y., Nomiya,M., Yamaguchi,O., Gotanda,K., Constantinou,C.E. Functional and anatomical effects of hormonally induced experimental prostate growth: A urodynamic model of benign prostatic hyperplasia (BPH) in the beagle. PROSTATE 2004; 58(-2-):156-163. >>> Dihydrotestosterone, 5 alpha-; estradiol, 17 beta; SC; Dog; 2ML4; 28 days; Animal info (Beagle, 3.5-7.2 yrs); testosterone induced BPH animal model.

P6588 Min,K., Munarriz,R., Kim,N.N., Choi,S., O'Connell,L., Goldstein,I., Traish,A.M. Effects of ovariectomy and estrogen replacement on basal and pelvic nerve stimulated vaginal lubrication in an animal model. JOURNAL OF SEX&MARITAL THERAPY 2003; 29(-1-):77-84. >>> Estradiol; PEG 300; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; replacement therapy (ovariectomy); post op. care (butorphanol tartrate, cephazolin).

P6480 Traish,A.M., Kim,N.N., Huang,Y.H., Min,K., Munarriz,R., Goldstein,I. Sex steroid hormones differentially regulate nitric oxide synthase and arginase activities in the proximal and distal rabbit vagina. International Journal of Impotence Research 2003; 15(-6-):397-404. >>> Testosterone; dehydroepiandrosterone; dihydrotestosterone, 5-alpha-; androstenediol, delta 5-3B, 17B; estradiol; progesterone; PEG; SC; Rabbit; 2002; 2 weeks; Controls received mp w/ vehicle; replacement therapy (ovariectomy); multiple pumps per animal (2).

P6109 Wang,X.X., Wu,J., Chiba,H., Umegaki,K., Yamada,K., Ishimi,Y. Puerariae radix prevents bone loss in ovariectomized mice. JOURNAL OF BONE AND MINERAL METABOLISM 2003; 21(-5-):268-275. >>> Estradiol, 17B; SC; Mice; 4 weeks; Replacement therapy (ovariectomy); pump model not stated.

P5951 Comeau,L.A., Campana,S.E. Modifying thyroidal status in Atlantic cod by osmotic pump delivery of thyroid and antithyroid agents. TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY 2003; 132(-5-):1021-1026. >>> Triiodothyronine; iodide, potassium; methimazole; estradiol, 17B; testosterone; thiourea; Saline; IP; Fish (cod); 2ML1; 17 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; drug plasma levels taken; potassium iodide, methimazole and thiourea are thyroid inhibitors; sex hormones were in a separate study where the ALZET pump model was not listed; "this study demonstrates the value of osmotic pumps as effective delivery vehicles for drugs in wild demersal fish." p. 1024.

P5931 Momoi,H., Ikomi,F., Ohhashi,T. Estrogen-induced augmentation of endothelium- dependent nitric oxide-mediated vasodilation in isolated rat cerebral small arteries. Japanese Journal of Physiology 2003; 53(-3-):193-203. >>> Estradiol, 17B; Polypropylene glycol; SC; Rat; 2002; 7 days; Replacement therapy (ovariectomy).

P5927 Shao,T.C., Li,H.L., Eid,W., Ittmann,M., Unni,E., Cunningham,G.R. In vivo preservation of steroid specificity in CWR22 xenografts having a mutated androgen receptor. PROSTATE 2003; 57(-1-):1-7. >>> Testosterone; estradiol; progesterone; flutamide; Cylodextrin, 2-beta-hydroxypropl; SC; Mice (nude); 2004; 4 weeks; Replacement therapy (castration); cancer (prostate); CWR22 xenograft used; flutamide is an anti-androgen; animal info (5-6 week old, nude ,ORX).

P5894 Jalouli,M., Carlsson,L., Ameen,C., Linden,D., Ljungberg,A., Michalik,L., Eden,S., Wahli,W., Oscarsson,J. Sex difference in hepatic peroxisome proliferator-activated receptor alpha expression: Influence of pituitary and gonadal hormones. Endocrinology 2003; 144(-1-):101-109. >>> Growth hormone, bovine; testosterone; estradiol, 17B-; Phosphate buffer; glycerol; sodium asize; propylene glycol; SC; Rat; 2001; 7 days; Replacement therapy (gonadectomy); comparison of daily injections vs. chronic mp; peptides; GH was recomb bovine&diluted in 0.05 m phosphate buffer, ph 8.6, with 1.6% glycerol&0.02% sodium azide; testosterone&estradiol were diluted in propylene glycol.

P5855 Wu,J., Wang,X.X., Chiba,H., Higuchi,M., Takasaki,M., Ohta,A., Ishimi,Y. Combined intervention of exercise and genistein prevented androgen deficiency-induced bone loss in mice. Journal of Applied Physiology 2003; 94(-1-):335-342. >>> Genistein; Estradiol, 17B-; DMSO; PEG 300; SC; Mice; 2002; 4 weeks; Replacement therapy (orchidectomy); dose-response (p.336); 20% DMSO used in vehicle.

P5732 Sims,N.A., Clement-Lacroix,P., Minet,D., Fraslon-Vanhulle,C., Gaillard-Kelly,M., Resche-Rigon,M., Baron,R. A functional androgen receptor is not sufficient to allow estradiol to protect bone after gonadectomy in estradiol receptor-deficient mice. Journal of Clinical Investigation 2003; 111(-9-):1319-1327. >>> Estradiol, 17B-; SC; Rat; 2ML4; ; Replacement therapy (ovariectomy).

P6166 Min,K., Munarriz,R., Kim,N.N., Goldstein,I., Traish,A. Effects of ovariectomy and estrogen and androgen treatment on sildenaf il-mediated changes in female genital blood flow and vaginal lubricati on in the animal model. American Journal of Obstetrics and Gynecology 2002; 187(-5-):1370-1376. >>> Estradiol; testosterone; PEG; SC; Rabbit; 2002; 14 days; Controls received mp w/ vehicle; estradiol plasma levels taken; replacement therapy (ovariectomy); multiple pumps per animal used (2) for estradiol and testosterone group; animal info (female, New Zealand, white, 3.5-4.0 kg).

P5853 Golding,E.M., Ferens,D.M., Marrelli,S.P. Altered calcium dynamics do not account for attenuation of endothelium-derived hyperpolarizing factor-mediated dilations in the female middle cerebral artery. Stroke 2002; 33(-12-):2972-2977. >>> Estradiol, 17B-; DMSO; NaCl; SC; Rat; 2004; ; Functionality of mp verified by 17ß-Estradiol plasma levels (p.2974); replacement therapy (ovariectomy); 50% DMSO used.

P5431 Mukundan,H., Resta,T.C., Kanagy,N.L. 17beta-Estradiol decreases hypoxic induction of erythropoietin gene expression. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2002; 283(-2-):R496-R504. >>> Estradiol, 17B; Polypropylene glycol; SC; Rat; 2002; 14 days; Controls received mp w/ vehicle; replacement therapy (ovariectomy).

P5401 Van Abel,M., Hoenderop,J.G.J., Dardenne,O., St Arnaud,R., Van Os,C.H., Van Leeuwen,H.J.P.T., Bindels,R.J.M. 1,25-dihydroxyvitamin D-3-independent stimulatory effect of estrogen on the expression of ECaC1 in the kidney. Journal of the American Society of Nephrology 2002; 13(-8-):2102-2109. >>> Estradiol, 17B-; DMSO; ethanol; water; SC; Mice; 1007D; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; vehicle composed of 50% DMSO, 15% ethanol; 35% water.

P5399 Tofovic,S.P., Dubey,R., Salah,E.M., Jackson,E.K. 2-hydroxyestradiol attenuates renal disease in chronic puromycin aminonucleoside nephropathy. Journal of the American Society of Nephrology 2002; 13(-11-):2737-2747. >>> Estradiol, 2-hydroxy-; PEG 400; Rat; 2ML4; 12 weeks.

P5377 Liang,Y.Q., Akishita,M., Kim,S., Ako,J., Hashimoto,M., Iijima,K., Ohike,Y., Watanabe,T., Sudoh,N., Toba,K., Yoshizumi,M., Ouchi,Y. Estrogen receptor beta is involved in the anorectic action of estrogen. International Journal of Obesity 2002; 26(-8-):1103-1109. >>> Estradiol, 17B-; Oligonucleotide, antisense; Cyclodextrin, 2-hydroxypropyl-beta (molecusol)-; fluorescein isothiocyanate; CSF/CNS; Rat; 2002; 3 weeks; Replacement therapy (ovariectomy); pumps replaced after one week; ALZET brain infusion kit used; initial pump delivered saline only for one week to allow for surgical recovery prior to 2 weeks of hormone administration.

P5347 Ishimi,Y., Yoshida,M., Wakimoto,S., Wu,J., Chiba,H., Wang,X., Takeda,K., Miyaura,C. Genistein, a soybean isoflavone, affects bone marrow lymphopoiesis and prevents bone loss in castrated male mice. Bone 2002; 31(-1-):180-185. >>> Genistein; estradiol, 17B-; DMSO; PEG 300; SC; Mice; 2002; ; Controls received mp w/ vehicle.

P5164 Wang,X., Abdel-Rahman,A.A. Estrogen modulation of eNOS activity and its association with caveolin-3 and calmodulin in rat hearts. American Journal of Physiology-Heart and Circulatory Physiology 2002; 282(--):H2309-H2315. >>> Estradiol, 17B-; Saline; SC; rat; 2004; 12 weeks; controls received mp w/ vehicle; functionality of mp verified by plasma estradiol levels; replacement therapy (ovariectomy); long-term study, pumps replaced every 4 wks; cardiovascular.

P5163 Earley,S., Resta,T.C. Estradiol attenuates hypoxia-induced pulmonary endothelin-1 gene expression. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY 2002; 283(--):L86-L93. >>> Estradiol, 17B-; Propanediol, 1,2-; Ethanol; SC; rat; 2002; 2ML4; 4 weeks; 24 hours; controls received mp w/ vehicle; replacement therapy (ovariectomy, p. L87); vehicle was 97% 1,2-propanediol and 3% ethanol; 2002 used for 24 hr infusion.

P5138 Kawamoto,S., Ejiri,S., Nagaoka,E., Ozawa,H. Effects of oestrogen deficiency on osteoclastogenesis in the rat periodontium. Arch Oral Biol 2002/1; 47(--):67-73. >>> Estradiol, 17B-; Propylene glycol; SC; rat; 2002; 7 or 14 days; controls received mp w/ vehicle; replacement therapy (ovariectomy p. 68).

P5120 Sahlin,L., Elger,W., Hedden,A., Lindberg,M., Reddersen,G., Schneider,B., Schwarz,S., Freyschuss,B., Eriksson,H. Effects of estradiol and estradiol sulfamate on the liver of ovariectomized or ovariectomized and hypophysectomized rats. Journal of Steroid Biochemistry and Molecular Biology 2002; 80(--):457-467. >>> Estradiol, 17B-; Estradiol sulfamate; Dexamethasone; Growth hormone, human; Propylene glycol; SC; rat; 2001; 7 days; controls received mp w/ vehicle; replacement therapy (ovariectomy, hypophysectomy, p. 458); comparison of oral administration vs. mp; steroid administered in PG, human GH pre-formulated.

P4985 Mills,L.H., Lee,A.J., Parlow,A.F., Zhu,B.T. Preferential growth stimulation of mammary glands over uterine endometrium in female rats by a naturally occurring estradiol-17 beta-fatty acid ester. Cancer Research 2001; 61(--):5764-5770. >>> Estradiol, 17-beta; Estradiol, 17-beta-S; Ethanol; DMSO; SC; rat; 1002; 2004; 10 or 23 days; controls received mp w/ vehicle; functionality of mp verified by plasma estradiol levels; replacement therapy (ovariectomy); dose-response (table 1 p. 5768); cancer; vehicle was 70% ethanol, 30% DMSO; 2nd experiment used 2004 pumps for 23 days;.

P4938 Cabot,C., Grasa,M.D.M., Massanes,R.M., de Matteis,R., Cinti,S., Fernandez-Lopez,J.A., Remesar,X., Alemany,M. Oleoyl-estrone does not have direct estrogenic effects on rats. LIFE SCIENCES 2001; 69(--):749-761. >>> Estrone, oleoyl-; Liposome suspension; IV (jugular); rat; 2ML2; 3, 6, 10 days; controls received mp w/ vehicle; functionality of mp verified by plasma estrone levels; comparison of oral gavage vs. mp; dose-response (p. 756); liposome suspension contained 20% lipid; "We found that acyl-estrone concentrations in plasma were in a similar range using either IV or oral administration; however, in the IV treatment, free estrone levels were 3.5-fold higher and those of B-estradiol were 4-fold higher than the corresponding values found in orally-treated animals". p. 753-54;.

P4901 Heaney,A.P., Nelson,V., Fernando,M., Horwitz,G. Transforming events in thyroid tumorigenesis and their association with follicular lesions. Journal of Clinical Endocrinology and Metabolism 2001; 86(-10-):5025-5032. >>> Estradiol, 17B-; Tamoxifen, 4-hydroxy-; PEG; Ethanol; SC; rat; ; Functionality of mp verified by serum estrogen levels; replacement therapy (ovariectomy); vehicle was 90% polyethylene glycol, 10% ethanol; 100 ul pumps used, no model or duration specified..

P4689 Kinuta,K, Tanaka,H, Moriwake,T, Aya,K, Kato,S, Seino,Y  Endocrinology 2000; 141(-4-):1317-1324. >>> Estradiol, 17B-;; mice;; 5 weeks;; Functionality of mp verified by serum estradiol level; comparison of IP injections vs. mp; pumps implanted in 5 - 10 week old mice;.

P4635 Zhang,L., Fishman,M.C, Huang,P.L  Arterioscler.Thromb.Vasc.Biol. 1999; 19(--):2059-2065. >>> Estradiol, 17B-; Progesterone; Gonadotrophin, human chorionic; SC; Mice; 2002; 14 days; Controls received sham operation; functionality of mp verified by serum levels; replacement therapy (ovariectomy); vascular cuff injury; B human chorionic gonadotrophin used;.

P4598 Bartholomeusz,R.K, Bruce,N.W., Lynch,A.-M  Biology of Reproduction 1999; 61(--):46-50. >>> Estradiol, 17B-;; Ethanol; Propylene glycol;; SC;; rat;; 2001;; ; controls received mp w/vehicle ; functionality of mp verified by plasma levels;.

P4488 Ishimi,Y., Miyaura,C, Ohmura,M, Onoe,Y, Sato,T, Uchiyama,Y, Ito,M, Wang,X, Suda,T, Ikegami,S  Endocrinology 1999; 140(-4-):1893-1900. >>> Estradiol, 17 B-; Genistein; DMSO; PEG 300; SC; Mice; 2 - 4 weeks; controls received mp w/vehicle; replacement therapy (ovariectomy); genistein is an isoflavone present in soybeans; structure is similar to estrogen;.

P4468 Qian,H, Barb,C.R, Compton,M.M, Hausman,G.J., Azain,M.J., Kraeling,R.R, Baile,C.A  Domest.Anim.Endocrinol. 1999; 16(-2-):135-143. >>> Estradiol benzoate;; Polypropylene glycol;; SC;; pig;; 2ML2;; 7 days;; controls received mp w/vehicle;.

P5561 Kraeling,R.R., Johnson,B., Barb,C.R., Rampacek,G.B. Evidence for a luteinizing hormone surge center in the hypothalamus of the pig. Biol.Reprod. 1998/5; 58(-5-):1199-1205. >>> Estradiol benozoate; Polypropylene glycol; SC; Pig; 2ML2; 7 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels (Fig. 3, p. 1201); 2ML2 pumps deliver 120 ul per day, not 2ML per day as mentioned in paper.

P4639 Adan,C., Cabot,C., Vila,R., Grasa,M.M., Masanes,R.M, Esteve,M, Estruch,J., Fernandez-Lopez,J.A., Remesar,X., Alemany,M. Oleoyl-estrone treatment affects the ponderostat setting differently in lean and obese Zucker rats.. International Journal of Obesity 1998; 23(--):366-373. >>> Estrone, oleoyl-;; Liposomes;; SC;; rat;; 2ML2;; 14 days;; controls received mp w/vehicle; short polyethylene capillary tube was used;.

P4153 Grasa,M.M., Cabot,C., Adan,C., Sanchis,D., Balada,F., Estruch,J., Fernandez-Lopez,J.A., Remesar,X., Alemany,M. Effect of oleoyl-estrone administration on corticosterone binding to tissues of lean and obese Zucker rats. J.Steroid Biochem.Molec.Biol. 1998; 66(-3-):165-169. >>> Estrone, oleoyl-; Liposomes; IV (jugular); rat; 2ML2; 14 days; controls received mp w/liposomes; oleoyl-estrone incorporated into liposomes is referred to as "Merlin-2"; PE10 catheter used.

P4152 Cabot,C., del Mar Grasa,M., Estruch,J., Fernandez-Lopez,J.-A., Remesar,X., Alemany,M. Zucker obese rats are insensitive to the CRH-increasing effect of oleoyl-estrone. Brain Res.Bull. 1998; 46(-6-):529-534. >>> Estrone, oleoyl-; Liposomes; IV (jugular); rat; 2ML2; 3,6,10,14 days; controls received mp w/liposome solution.

P4151 Cros,M., Silve,C., Graulet,A.-M., Morieux,C., Urena,P., de Vernejoul,M.-C., Bouizar,Z. Estrogen stimulates PTHrP but not PTH/PTHrP receptor gene expression in the kidney of ovariectomized rat. J.Cell.Biochem. 1998; 70(--):84-93. >>> Estradiol, 17B; DMSO; Saline; SC; rat; 14 days; controls received mp w/vehicle or sham operation; functionality of mp verified by plasma levels; replacement therapy (ovariectomy).

P4456 Masamura,S, Santner,S.J, Gimotty,P, George,J, Santen,R.J. Mechanism for maintenance of high breast tumor estradiol concentrations in the absence of ovarian function: Role of very high affinity tissue uptake.. Breast Cancer Res.Treat. 1997; 42(--):215-226. >>> Estradiol, 17B-;; PEG;; SC;; rat;; 2ML2;; 2 weeks;; controls received mp w/vehicle; functionality of mp verified by plasma levels; replacement therapy ( ovariectomy); dose-response (p. 218); cancer;.

P4146 Balada,F., Sanchis,D., Virgili,J., Grasa,M.M., Monserrat,C., Fernandez-Lopez,J.A., Remesar,X., Alemany,M. Effect of the slimming agent oleoyl-estrone in liposomes on the body weight of rats fed a cafeteria diet. Arch.Physiol.Biochem. 1997; 105(-5-):487-495. >>> Estrone, oleoyl-; Liposomes; IV (jugular); rat; 2ML2; 28 days; controls received mp w/liposome suspension; pumps replaced after 14 days; oleoyl-estrone in liposomes was named Merlin-2.

P3966 Saito,S., Motomura,N., Lou,H., Ramwell,P.W., Foegh,M.L. Specific effects of estrogen on growth factor and major histocompatibility complex class II antigen expression in rat aortic allograft. J.Thorac.Cardiovasc.Surg. 1997; 114(--):803-810. >>> Estradiol, 17 B; SC; rat; 3, 5, 9, 16, 23 days; controls received placebo; functionality of mp verified by estradiol serum assays; immunology; cardiovascular.

P3964 Jahangiri,L., Kim,A., Nishimura,I. Effect of ovariectomy on the local residual ridge remodeling. J.Prosthet.Dent 1997; 77(--):435-443. >>> Estradiol, 17B; rat; 2002; no duration posted; controls received mp w/vehicle; replacement therapy (ovariectomy); no stress (see pg. 438-9).

P3831 Balada,F., Sanchis,D., Grasa,M.M., Virgili,J., Estruch,J., Fernandez-Lopez,J.-A., Remesar,X., Alemany,M. Effect of the slimming agent oleoyl-estrone in liposomes on the body weight of zucker obese rats. Int.J.Obes. 1997; 21(--):789-795. >>> Estrone, oleoyl-; Liposomes; IV (left jugular); rat; 2ML2; 28 days; controls received mp w/liposomes; pumps replaced after 2 weeks; stress/adverse reaction: transient weight loss after surgical implantation of mp (pg. 790); oleoyl-estrone in liposomes referred to as "merlin-2".

P3748 Shetty,G., Krishnamurthy,H., Krishnamurthy,H.N., Ramachandra,S.G., Moudgal,N.R. Use of norethisterone and estradiol in mini doses as a contraceptive in the male. Contraception 1997; 56(--):257-265. >>> Estradiol benzoate; Norethisterone; DMSO; Propylene glycol; monkey; 4, 8 weeks; comparison of im injections vs. mp; agents given singly and in combination in the same pump; "studies w/ ALZET minipump have shown that, by using a constant daily release system, the effective dose of steroid combination can be minimized." (p. 262).

P3745 Sanchis,D., Adan,C., Ardevol,A., del Mar Grasa,M., Cabot,C., Balada,F., Vila,R., Estruch,J., Puerta,M., Fernandez-Lopez,J.-A., Remesar,X., Alemany,D.M. Short-term treatment with oleoyl-oestrone in liposomes (Merlin-2) strongly reduces the expression of the ob gene in young rats. Biochem.J. 1997; 326(--):357-360. >>> Oestrone, oleoyl; Liposomes; IV (jugular); rat; 2ML2; 3, 6, 10, 14 days; controls received mp w/ liposomes; functionality of mp verified by radioimmunoanalysis of 3H-oestrone; Merlin-2 is code name for oestrone, oleoyl in liposomes.

P6293 Lu,R., Kanai,N., Bao,Y., Wolkoff,A.W., Schuster,V.L. Regulation of renal oatp mRNA expression by testosterone. American Physiological Society 1996; (--):F332-F337. >>> Testosterone; estradiol; dexamethasone; PEG 600; SC; Rat; 2001; 1 week; Controls received mp w/ vehicle; replacement therapy (gonadectomy, adrenalectomy, orchidectomy, oophorectomy).

P3697 Maeda,T., Ikegami,H., Sakata,M., Yamaguchi,M., Wada,K., Koike,K., Adachi,K., Kurachi,H., Hirota,H., Miyake,A. Intraventricular administration of estradiol modulates rat prolactin secretion and synthesis. J.Endocrinol.Invest. 1996; 19(--):586-592. >>> Estradiol; Saline; CSF/CNS; rat; 2001; 1, 3, 7 days; controls received mp w/ saline; replacement therapy (ovariectomy); portion of connecting tube was left externally so a clip could shut off flow from mp to allow a few days recovery from surgery; sc E(2) pellets used prior to ICV pump; ALZET brain infusion kit used.

P3526 Sanchis,D., Balada,F., del Mar Grasa,M., Virgili,J., Peinado,J., Monserrat,C., Fernandez-Lopez,J.-A., Remesar,X., Alemany,M. Oleoyl-estrone induces the loss of body fat in rats. Int.J.Obes. 1996; 20(--):588-594. >>> Estrone, oleoyl-; Liposomes; IV (jugular); rat; 2ML2; 14 days; no comment posted.

P3450 Schroder,J., Doren,M., Schneider,B., Oettel,M. Are the antioxidative effects of 17 b-estradiol modified by concomitant administration of a progestin?. Maturitas 1996; 25(--):133-139. >>> Estradiol, 17 B; Levonorgestrel; Propylene glycol; SC; rabbit; 1003D; 3 days; controls received mp w/ vehicle; replacement therapy (ovariectomy); estradiol given with and without concomitant levonorgestrel.

P3698 Nolan,C., Proietto,J. The effects of oophorectomy and female sex steroids on glucose kinetics in the rat. Diabetes Res.Clin.Pract. 1995; 30(--):181-188. >>> Estradiol, 17B-; Progesterone; Propylene glycol; SC; rat; 2ML1; 7 days; controls received sham oophorectomy and/or mp w/ vehicle; replacement therapy (oophorectomy).

P3385 Shetty,G., Bhatnagar,A.S., Moudgal,N.R. Blockade of estrogen synthesis with an aromatase inhibitor affects luteal function of the pseudopregnant rat. J.Steroid Biochem.Molec.Biol. 1995; 55(-3/4-):347-353. >>> Fadrozole HCl; Estradiol-3-benzoate; SC; rat; 2002; 3 days; no comment posted.

P2826 Kimble,R.B., Matayoshi,A.B., Vannice,J.L., Kung,V.T., Williams,C., Pacifici,R. Simultaneous block of interleukin-1 and tumor necrosis factor is required to completely prevent bone loss in the early postovariectomy period. Endocrinology 1995; 136(-7-):3054-3061. >>> Interleukin-1 receptor antagonist; Estradiol, 17B-; Albumin, bovine serum; SC; rat; 2002; 2 weeks; controls received sham operations and all agents but estradiol; replacement therapy (ovariectomy).

P3029 Vanderwall,D.K., Woods,G.L., Weber,J.A., Lichtenwalner,A.B. Corpus luteal function in nonpregnant mares following intrauterine administration of prostaglandin E2 or estradiol-17B. Theriogenology 1994; 42(--):1069-1083. >>> Prostaglandin E2; Estradiol, 17B-; Ethanol; PBS; intrauterine; horse; 2001; 7 days; controls received no treatment or mp with vehicle; tissue perfusion (uterine horn lumen); no stress (see pg. 1078); benzathine penicillin-G given prophylactically.

P2966 Sahlin,L., Norstedt,G., Eriksson,H. Estrogen regulation of the estrogen receptor and insulinlike growth factor-I in the rat uterus: a potential coupling between effects of estrogen and IGF-I. Steroids 1994; 59(--):421-430. >>> Insulin-like growth factor I; Estradiol, 17B-; SC; rat; 3 days; 0,21,69,93,168 hours; replacement therapy (ovariectomy); comparison of sc injections vs. vaginal implants vs. mp; peptides; recomb. human IGF-1 used.

P2293 Han,X., Liehr,J.G. DNA single-strand breaks in kidneys of syrian hamsters treated with steroidal estrogens: hormone-induced free radical damage preceding renal malignancy. Carcinogenesis 1994; 15(-5-):997-1000. >>> Estradiol; Propylene glycol; SC; hamster; 7 days; cancer.

P3238 Lobo,M.J., Remesar,X., Alemany,M. Effect of chronic intravenous injection of steroid hormones on body weight and composition of female rats. Biochem.Molec.Biol.Intl. 1993; 29(-2-):349-358. >>> Progesterone; Cortisol; Cortisone; Corticosterone; Dehydroepiandrosterone; Androstenedione, 4-; Androstendiol, 5-; Testosterone; Nortestosterone, 19-; Estradiol, B-; Estrone; Estriol; Deoxycorticosterone; PEG 400; IV (lower cava); rat; 2002; 15 days; controls received mp with PEG; no stress (see pg. 351); pumps placed into peritoneal cavity and sutured to musculature; surgical wound sprinkled with sulphathiazol.

P3233 Lee,M.-Y., Clark,J.E., Williams,D.E. Induction of flavin-containing monooxygenase (FMO B) in rabbit lung and kidney by sex steroids and glucocorticoids. Archives Biochem.Biophys. 1993; 302(-2-):332-336. >>> Estradiol, B-; Progesterone; Propylene glycol; Ethanol; Ascorbic acid; IP; rabbit; 2001; 4-5 days; controls received mp w/pg; functionality of mp verified by plasma levels; comparison of injections vs. mp.

P2634 Beattie,J.H. The metabolic fate of [3H]estradiol in relation to dietary intake of boron in ovariectomized rats. J.Steroid Biochem.Molec.Biol. 1993; 45(-6-):549-554. >>> Estradiol, 17B-; Radio-isotopes; 3H tracer; Ethanol; Albumin, rat serum, fatty-acid free; SC; rat; 2002; no duration posted; functionality of mp verified by residual volume; replacement therapy (ovariectomy).

P2376 Nagae,Y., Takahashi,S., Takahashi,M., Deguchi,J., Yasuda,S., Kawahara,M., Mori,T., Miyamoto,M. A 3-month subacute toxicity study on 17b-Estradiol by continuous infusion in ovariectomized rats. Jpn.Pharmacol.Ther. 1992; 20(-10-):37-50. >>> Estradiol, 17B-; SC; rat; 3 months; replacement therapy (ovariectomy); toxicology; in Japanese with English abstract.

P2112 Yamauchi,T., Akita,S., Noguchi,S., Inukai,T. The effect of continuous 17 B-estradiol infusion by osmotic minipump on estrous cycle, body weight and uterine weight. Jpn.Pharmacol.Ther. 1992; 20(-7-):107-112. >>> Estradiol, 17B-; rat; 2002; 14 days; functionality of mp verified by serum levels; replacement therapy (ovariectomy).

P3248 Lavia,L.A., Larson,B.A. Rat endometrial stromal-epithelial response to estrogen infusion. Steroids 1991; 56(--):123-130. >>> Estradiol; SC; rat; 2001; 4-96 hours; replacement therapy (ovariectomy).

P3253 LaPolt,P.S., Day,J.R, Lu,J.K.H. Effects of estradiol and progesterone on early embryonic development in aging rats. Biol.Reprod. 1990; 43(--):843-850. >>> Estradiol; Propylene glycol; SC; rat; 2ML4; no duration posted; functionality of mp verified by serum levels.

P2954 Santner,S.J., Levin,M.C., Santen,R.J. Estrone sulfate stimulates growth of nitrosomethylurea-induced breast carcinoma in vivo in the rat. Int.J.Cancer 1990; 46(--):73-78. >>> Estrone sulfate; PEG 600; 3H tracer; Radio-isotopes; SC; rat; 2 weeks; functionality of mp verified by serum levels; replacement therapy (ovariectomy); cancer.

P1652 Takano-Yamamoto,T., Rodan,G.A. Direct effects of 17B-estradiol on trabecular bone in ovariectomized rats. Proc.Natl.Acad.Sci. 1990; 87(--):2172-2176. >>> Estradiol, 17B-; Ethanol; Saline; bone (femur); rat; 2001; 8 days; tissue perfusion; replacement therapy (ovariectomy).

P1872 Lustig,R.H., Mobbs,C.V., Pfaff,D.W., Fishman,J. Temporal actions of 16a-hydroxyestrone in the rat: comparisons of lordosis dynamics with other estrogen metabolites and between sexes. J.Steroid Biochem. 1989; 33(-3-):417-421. >>> Estradiol metabolite; Estriol precursor; Estrone, 16 alpha hydroxy; Propylene glycol; SC; rat; 13 days; replacement therapy (ovariectomized).

P1632 Kono,S., Higa,H., Sunagawa,H. Hypocholesterolemic effect of long-term continuous administration of 2-methoxyestriol in dietary hypercholesterolemic rats. J.Clin.Biochem.Nutr. 1989; 6(--):49-56. >>> Estradiol, 17B-; Estriol, 2-methoxy-; Propylene glycol; SC; rat; 4 weeks; replacement therapy (oophorectomy); paper incorrectly states that the 2ML-size pump had a rate of 0.5 ul/hr.

P1271 Hatjis,C.G., Koritnik,D.R., Crews,A. Up-regulation of guinea pig myometrial b-adrenoreceptors by systemic estradiol and progesterone. Endocrinology 1988; 122(-4-):1455-1459. >>> Estradiol, 17B-; Progesterone; Propylene glycol; guinea-pig; 7 days; Pump model not stated; controls received mp w/vehicle; separate and simultaneous infusion of agents; replacement therapy (oophorectomy).

P1130 Carlsson-Bostedt,L., Frohlander,N., Eden,S., Stigbrand,T., von Schoultz,B. Effects of oestrogen and human growth hormone on pregnancy-associated plasma proteins in the rat. Acta Endocrinol. 1987; 116(--):299-304. >>> Estradiol, 17B-; Growth hormone, human; Ethanol; Glycerol; Saline; Water; SC; rat; 2002; 12 days; controls received mp w/ saline; saline is vehicle for hGH; concomitant and simultaneous infusion of agents; peptides.

P1054 Douglas,J.G. Estrogen effects on angiotensin receptors are modulated by pituitary in female rats. Am.J.Physiol. 1987; 252(--):E57-E62. >>> Aldosterone; Estradiol; PEG; IP; rat; 2001; 1-7 days; controls received mp w/aldosterone; agents infused together and/or seperately; replacement therapy (oophorectomy).

P3273 MacLusky,N.J., Krey,L.C., Parsons,B., Merriam,G.R., Loriaux,D.L., Pfeiffer,D.G., Naftolin,F. Are catechol oestrogens obligatory mediators of oestrogen action in the central nervous system? II. Potencies of natural and synthetic oestrogens for induction of gonadotrophin release and female sexual behaviour in the rat. J.Endocrinol. 1986; 110(--):499-505. >>> Estradiol, 17B-; Estradiol, 17A-; Estradiol, 2-fluoro-; Estradiol, 4-fluoro-; Moxestrol; Propylene glycol; SC; rat; 2001; 24 hours; controls received mp w/vehicle; replacement therapy (ovariectomy).

P0897 Forcelledo,M.L., Croxatto,H.B. Ovum transport in cyclic rats rendered hypo-oestrogenic by treatment with 4-hydroxy-4-androstene-3,17-dione. J.Reprod.Fertil. 1986; 76(--):325-330. >>> Estradiol; Propylene glycol; SC; rat; 2001; no duration posted; controls received mp w/vehicle; dose-response; one group treated w/testosterone via silastic capsules.

P0896 Lavia,L.A., Roberts,D.K., Walker,N.J., Anderson,K. Rat luminal cell nuclear area changes correlated with uterine growth responses induced by a low dose infusion or injection of estradiol-17B. Steroids 1986; 45(-6-):519-537. >>> Estradiol, 17B-; SC; rat; 2001; 4, 10, 18, and 41 hours; pumps primed in saline at 38C 24 hrs. prior to implant; comparison of ip injection vs. mp infusion.

P0764 Dey,S.K., Johnson,D.C., Pakrasi,P.L., Liehr,J.G. Estrogens with reduced catechol-forming capacity fail to induce implantation in the rat. Proc.Soc.Exp.Biol.Med. 1986; 181(--):215-218. >>> Estradiol, 4-hydroxy-; rat (pregnant); 24 hours; comparison of injections vs. mp infusion; injection of 2-fluroestradiol w/mp infusion of agent.

P0763 Panek,D.U., Dixon,W.R. Effect of continuous intraventricular estrogen or catechol estrogen treatment on catecholamine turnover in various brain regions. J.Pharmacol.Exp.Ther. 1986; 236(-3-):646-652. >>> Estradiol, 17A-; Moxestrol; Estradiol, 17B-; Estradiol, 2-hydroxy-; Estradiol, 4-hydroxy-; Estrone, 2-hydroxy-; Acetic acid; Ascorbic acid; Ethanol; Propylene glycol; CSF/CNS; rat; 2001; 7 days; comparison of agents effects; replacement therapy (ovariectomy); stability of CEs in mp; mp connected to stainless steel cannula in lateral ventricle via polyethylene tubing; mp primed in saline overnight.

P0687 Muller,R.E., Traish,A.M., Wotiz,H.H. Interaction of estradiol and estriol with uterine estrogen receptor in vivo and in excised uteri or cell suspensions at 37C: noncooperative estradiol binding and absence of estriol inhibition of estradiol-induced receptor activation and transformation. Endocrinology 1985; 117(-5-):1839-1847. >>> Estradiol; Estriol; Ethanol; Propylene glycol; Water; SC; rat; 2002; 16 days; replacement therapy (ovariectomy); pump replaced on day 12; agents admin singly and as a mixture; pump functionality verified&advantage of mp p. 1841.

P0664 Panek,D.U., Dixon,W.R. Effect of intraventricular administration of catechol estrogens on catecholamine content in various brain regions. Brain Research 1985; 340(-1-):87-90. >>> Estradiol benzoate; Estradiol, 2-hydroxy-; Estradiol, 4-hydroxy-; Estrone, 2-hydroxy-; Acetic acid, glacial; Ascorbic acid; Ethanol; Propylene glycol; CSF/CNS; rat; 2002; 1 or 2 weeks; comparison of agents effects.

P0645 Kantor,B.S., Dey,S.K., Johnson,D.C. Catechol oestrogen induced initiation of implantation in the delayed implanting rat. Acta Endocrinol. 1985; 109(-3-):418-422. >>> Estradiol, 17B-; Estradiol, 2-hydroxy-; Estradiol, 4-hydroxy-; Acetic acid; Ascorbic acid; Ethanol; Propylene glycol; SC; rat; 2001; 6, 24 hours; comparison of sc injec vs. mp infusion; comparison of agents effects; replacement therapy (hypophysectomy); dose-response data; comparison of single injection vs. mp;.

P0566 Will,P.C., Cortright,R.N., DeLisle,R.C., Douglas,J.G., Hopfer,U. Regulation of amiloride-sensitive electrogenic sodium transport in the rat colon by steroid hormones. Am.J.Physiol. 1985; 248(-1-):G124-G132. >>> Aldosterone; Corticosterone; Dexamethasone phosphate; Estradiol, 17B-; Progesterone; Testosterone; PEG 400; PEG 600; IP; rat; 1701; 2001; 3-8 days; comparison of agents effects; replacement therapy (adrenalectomy&ovariectomy); controls received mp with solvent or glass rods of mp size; no stress implied G125, weight regained; functionality of mp verified.

P1224 Lavia,L.A., Shideler,C., Farley,N., Walker,N., Fields,W., Roberts,D.K. Uterine growth responses of the mature castrate rat to estradiol-17B. Steroids 1984; 43(-6-):663-675. >>> Estradiol, 17B-; Progesterone; SC; rat; 2001; 24 hours; separate and simultaneous infusion of agents; 2 doses of E2 infused; functionality of mp verified by radioimmunoassay.

P0284 Ball,P., Emons,G., Haupt,O., Knuppen,R. Pharmacological effects of 2- and 4-methyloestradiol as a probe to test the biological importance of 2- and 4-hydroxylation of oestrogens (catecholoestrogen-formation). Acta Endocrinol. 1983; 102(--):150-152. >>> Catecholestrogens; Estradiol, 17B-; Acetic acid; Ascorbic acid; Ethanol; Propylene glycol; SC; rat; 2001; 3 days; replacement therapy (ovariectomy).

R0052 Ball,P., Emons,G., Knuppen,R. Importance of catecholestrogens in the regulation of the ovarian cycle. Arch.Gynecol. 1982; 231(--):315-320. >>> Estrogens, 2-hydroxy-; Estrogens, 4-hydroxy-; SC; rat; 152 hours; no comment posted.

P0238 Jellinck,P.H., Luine,V., McEwen,B.S. Differential effects of catechol estrogens, progestins and CI-628 administered by constant infusion on the central and peripheral action of estradiol. Neuroendocrinology 1982; 35(--):73-78. >>> CI-628; Progestins; R-5020; Catecholestrogens; Estradiol, 17B-; Progesterone; Ascorbic acid; PEG; SC; rat; 2001; 2-3 days; organ replacement therapy (ovariectomy); agents used alone and/or in combination; Cl-628 is an anti-estrogen; R-5020 is a progestin.

P0169 Emons,G., Knuppen,R., Ball,P. 4-hydroxyestradiol-17B and 4-hydroxyestriol-17a: comparative studies on central and peripheral effects of two epimeric catecholestrogens. Endocrinology 1981; 109(-5-):1799-1801. >>> Catecholestrogens; Acetic acid; Ascorbic acid; Ethanol; Propylene glycol; SC; rat; 2001; 3 days; no comment posted.

P0141 Naish,S.J., Ball,P. Catecholestrogens and induction of sexual behavior in the ovariectomized rat. Neuroendocrinology 1981; 32(--):225-228. >>> Catecholestrogens; Estradiol, 17B-; Acetic acid; Ascorbic acid; Ethanol; Propylene glycol; SC; rat; 1701; 7 days; comparison of acute iv injec. vs sc injec. or infusion; replacement therapy (ovariectomy); separate&simultaneous infusion of agents.

P0137 Ball,P., Emons,G., Gethmann,U. Effect of low doses of continuously administered catecholoestrogens on peripheral and central target organs. Acta Endocrinol. 1981; 96(-4-):470-474. >>> Catecholestrogens; Estradiol, 17B-; Acetic acid; Ascorbic acid; Ethanol; Propylene glycol; SC; rat; 1701; 152 hours; no comment posted.

P0125 Jellinck,P.H., Krey,L., Davis,P.G., Kamel,F., Luine,V., Parsons,B., Roy,E.J., McEwen,B.S. Central and peripheral action of estradiol and catecholestrogens administered at low concentration by constant infusion. Endocrinology 1981; 108(-5-):1848-1854. >>> Catecholestrogens; Estradiol, 17B-; Ascorbic acid; Propylene glycol; SC; rat; 1701; 55 hours - 3 days; organ replacement therapy (ovariectomy).

P0095 Chappel,S.C., Resko,J.A., Norman,R.L., Spies,H.G. Studies in rhesus monkeys on the site where estrogen inhibits gonadotropins: delivery of 17B-estradiol to the hypothalamus and pituitary gland. J.Clin.Endocrinol.Metab. 1981; 52(-1-):1-8. >>> Estradiol, 17B-; Radio-isotopes; 3H tracer; Ethanol; Saline; CSF/CNS; CSF/CNS (anterior pituitary); monkey; 18 hours; mp model not stated; comparison of site of administration; replacement therapy (ovariectomy).

P0074 Fishman,J., Martucci,C. Biological properties of 16alpha-hydroxyestrone: implications in estrogen physiology and pathophysiology. J.Clin.Endocrinol.Metab. 1980; 51(-3-):611-615. >>> Estrogen, 16a-; Catecholestrogens; Estradiol, 17B-; Estriol; Estrone; Estrone, 2-methoxy-; Ascorbic acid; Propylene glycol; SC; rat; 1, 2, or 3 days; organ replacement therapy (ovariectomy).

P0061 Will,P.C., Cortright,R.N., Hopfer,U. Polyethylene glycols as solvents in implantable osmotic pumps. J.Pharm.Sci. 1980; 69(-6-):747-749. >>> Aldosterone; Corticosterone; Deoxycorticosterone acetate; Dexamethasone acetate; Estradiol, 17B-; Hydrocortisone; Progesterone; Spironolactone; Testosterone; PEG; PEG 400; PEG 600; IP; rat; 1701; no duration posted; 3-7 days aldosterone, 6 days PEG only; replacement therapy (adrenalectomy).

P0041 Martucci,C.P., Fishman,J. Impact of continuously administered catechol estrogens on uterine growth and luteinizing hormone secretion. Endocrinology 1979; 105(-6-):1288-1292. >>> Catecholestrogens; Estradiol, 17B-; Ascorbic acid; Propylene glycol; SC; rat; 1, 2, or 3 days; comparison of single injection vs. infusion; organ replacement therapy (ovariectomy).

P0012 Butcher,R.L., Inskeep,E.K., Pope,R.S. Plasma concentrations of estradiol produced with two delivery systems in ovariectomized rats. Proc.Soc.Exp.Biol.Med. 1978; 158(-3-):475-477. >>> Estradiol, 17B-; Propylene glycol; SC; Rat; 1701; 1 week; 1-2 pumps/animal; comparison of injections vs. infusion; organ replacement therapy (ovariectomy).

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